ERcodon325、PR＋331G／A与乳腺癌关系的病例对照研究

A case-control study on the associations of ER codon 325 and PR +331G/A with the risk of breast cancer

目的：研究雌激素受体（estrogen receptor，ER）codon325、孕激素受体（progestin receptor，PR）＋331G／A基因与乳腺癌的关系以及它们与环境危险因素对乳腺癌的联合作用。方法：采用病例一对照研究的方法，收集206例乳腺癌新发病例，按年龄频数（±3岁）匹配214例健康对照女性。采用结构式调查表收集研究对象的流行病学资料，并采集3mL静脉血。应用荧光定量PCR法检测ERcodon325、PR＋331G／A的基因型。结果：与CC相比，ERcodon325位点CG和GG基因型的调整OR分别为0．99（95％CI：0．61～1．61）和1．13（95％CI：0．62～2．04）；与础相比，PR＋331G／A位点GG基因型的调整OR为1．51（95％CI：O．74—3．07），未发现有增加乳腺癌危险的联合作用（趋势检验：P=0．4090）；与无自然流产史且ERcodon325位点携带CC基因型的妇女相比，有自然流产史且携带CG和GG基因型的妇女OR为3．57（95％CI：1．21—10．52），相加模型检验接近有统计学意义（S=3．06，AP=0．97，u=1．8981，P=0．0588）。结论：ERcodon325、PR＋331G／A单基因多态性可能与乳腺癌发病风险无关，ERcodon325多态性与自然流产史具有增加乳腺癌危险的联合作用趋势。

Objective：To investigate the association of estrogen receptor （ER） codon 325 and progestin receptor （PR） ＋331G/A gene with the risk of breast cancer and the influence of the two genes combined with the environmental risk factors on breast cancer. Methods ： A case-control study was performed. Two hundred and six cases with newly diagnosed breast cancer were collected. Two hundred and fourteen healthy women were matched according to age frequency （ ±three years）. The epidemiological data were collected by questionnaire using structured tables. The 3 mL venous blood was collected and the genotypes of ER codon 325 and PR ＋ 331G/A were determined by fluorescent quantitative PCR. Results： Compared with CC, the adjusted OR was 0.99 （95% CI：0. 61-1.61 ） and 1. 13 （95% CI：0. 62-2.04）for CG genotype and GG genotype of ER-eodon 325, respectively. The adjusted OR was 1.51 （95% CI： O. 74- 3.07 ） for GG genotype of PR ＋ 331G/A. No synergistic effects of ER codon 325 and PR ＋ 331 G/A were observed in increasing the risk of breast cancer （ Trend analysis, P = 0. 409）. The adjusted OR was 3.57 （95% CI： 1.21-10.52） for the ER codon 325 CG- and GG-0 genotyped women with spontaneous abortion history compared with that in CC-gcnotypcd women without spontaneous abortion history. Additive models test demonstrated that the difference tended to be significant （ S = 3.06, AP = 0.97, u = 1. 898 1, P = 0. 058 8 ）. Conclusion：The single nucleotide polymorphism （SNP） of ER eodon 325 and PR ＋ 331 G/A genes are not associated with the risk of breast cancer. The SNP of ER codon 325 with spontaneous abortion history tends to synergistically increase the risk of breat cancer.