结膜下注射CTGF抗体对兔青光眼滤过手术后眼压和滤过泡面积的影响

Measurements of the filtering bleb area and intraocular pressure following subconjunctival injection of CTGF antibody after glaucoma filtration surgery in a rabbit model

目的:探讨结缔组织生长因子(connective tissue growth factor,CTGF)抗体对兔青光眼滤过手术后滤过泡瘢痕化的抑制作用。方法:家兔5只双眼制作青光眼滤过手术模型。随机选取家兔一眼作为抗体组,分别于手术完成当时和术后5d结膜下注射0.1mL浓度为50mg/L的CTGF抗体;另一眼作为对照组在相同时间点结膜下注射0.1mL磷酸盐缓冲液。术后1,3,5,7,10,14d分别观察滤过泡形态并测量其面积和眼压值。结果:术后7,10和14d抗体组滤过泡面积均大于对照组(P<0.05),眼压均小于对照组(P<0.05)。结论:结膜下注射CTGF抗体可维持兔眼滤过手术后较大的滤过泡面积和较低的眼压。

AIM： To study the role of connective tissue growth factor （CTGF） antibody in inhibiting bleb scarring after glaucoma filtration surgery （GFS） in a rabbit model. METHODS： GFS was performed on both eyes in 5 rabbits. One eye of each rabbits was chosen randomly as antibody group and received subconjunctival injection of 0.1mL CTGF antibody （50mg/L） when GFS finished and on 3 days after GFS. The other eye of each rabbit as control group was received subconjunctival injection of 0. 1mL phosphate buffered saline （PBS） at the same time as antibody group. On postoperative 1,3, 5, 7, 10 and 14 days, the appearance of filtrating blebs was observed under slit lamp, their areas and the intraocular pressure （IOP） were measured with micrometer and applanation tonometer, respectively. RESULTS： On postoperative 7, 10 and 14 days, areas of filtrating blebs in antibody group were all larger than that in control group （ P 〈 0.05） and lOPs of antibody group were lower than that of control group（ P〈 0.05）. CONCLUSION： Subconjunctival injection of CTGF antibody can maintain larger bleb area and lower IOP after GFS in rabbit. This indicates that CTGF antibody is most likely to be a novel agent to prevent excessive scarring following GFS.