摘要
目的建立人结肠癌HT-29裸鼠肝转移模型,检测肝脏组织中人端粒酶逆转录酶(hTERT)mRNA的表达。方法Balb/c裸鼠30只,腹腔内注射戊巴比妥麻醉,经脾脏注入人结肠癌HT-29细胞悬液,并切除脾脏,建立裸鼠肝转移模型。处死动物后观察肿瘤生长情况,并作病理学检查;采用RT-PCR方法检测裸鼠肝脏组织中hTERT mRNA的表达。结果30只裸鼠均发生人结肠癌肝转移,肝转移率100%,病理学证实肝转移发生,肝脏组织中hTERTmRNA表达阳性。结论脾种植HT-29细胞建立人结肠癌裸鼠肝转移模型,肝脏组织中hTERTmRNA阳性表达,提示hTERT在结肠癌肝转移早期诊治中的意义。
Objective To develop the human colon carcinoma (HT-29) liver metastasis model in nude mice, and to detect the expression of human telomerase reverse transcriptases (hTERT) mRNA in liver of nude mice. Methods Liver metastases were established in 30 Balb/c nude mice by intrasplenic injection of colonic cancer cells (HT-29) , and the spleens were reseeted. After being sacrificed, the tumor growth was observed, and the pathological examinations were performed. The expression of hTERT mRNA in the livers of nude mice was detected by RT-PCR technique. Results Human colon carcinoma (HT-29) liver metastasis model was developed in all the 30 nude mice, with the liver metastasis rate of 100%. The pathological results revealed the occurrence of liver metastases, and the expression of hTERT mRNA was positive in the liver tissues. Conclusion Intrasplenic injection of HT-29 cell is a reliable way for producing colonic cancer liver metastasis model, and the positive expression of hTERT mRNA in liver tissues indicates the significance of hTERT in the early diagnosis and treatment of colon carcinoma liver metastasis.
出处
《上海交通大学学报(医学版)》
CAS
CSCD
北大核心
2008年第10期1266-1268,共3页
Journal of Shanghai Jiao tong University:Medical Science
基金
上海市卫生局基金(05905)~~
关键词
结直肠癌
端粒酶逆转录酶
肝转移
裸鼠
colorectal cancer
telomerase reverse transcriptase
liver metastasis
nude mice