细胞周期素D1基因多态性与胃癌关系的研究

Study on the relationship between cyclin D1 G870A polymorphism and gastric cancer

目的研究与肿瘤发生有关的细胞周期蛋白D1（CCND1）870A／G基因多态性与胃癌易感性的关系。方法收集106例胃癌病例（包括胃体癌及胃窦癌）和108例非溃疡性消化不良患者的外周血白细胞，采用病例对照分子流行病学研究方法，以聚合酶链反应-限制性片段长度多态性（PCR—RFLP）技术，进行CCND1基因型检测。结果在病例组和对照组中CCND1 870位点野生型GG频率分别为19．8％和7．4％，携带AA基因型个体发生胃癌危险是携带GG型个体的0．28倍（P〈0．05，OR=0．281），即GG型个体更易感胃癌。分层分析表明，携带GG基因型个体分别在男性、年龄≥60岁及幽门螺杆菌感染亚组中患胃癌风险更高。结论在中国胃癌高发区西安，CCND1 870A／G位点GG基因型人群更易感胃癌。

Objective To assess whether the G870A polymorphism of cyclin D1 （CCND1）was associated with individual susceptibility to gastric cancer in China. Methods Case-control study of 214 individuals was conducted, including 108 controls （non ulcerative dyspepsia） and 106 cases of gastric cancer. The polymorphism analysis of CCND1 A/G was performed in blood samples by PCR-RFLP method. Results The frequency of GG genotype was 19.8% and 7.4% in patients with gastric cancer and controls, respectively. The risk for gastric cancer in patients homozygous （GG） for CCND1 genotypes was 3. 5 times higher in comparison with patients carrying AA genotypes （P〈 0. 05, OR = 0. 281）. Furthermore, the stratification analyses showed that the male, elderly （≥60 years） or H. pylori infected gastric cancer patients who carrying GG genotype were more susceptible to gastric cancer compared with those carrying AA or GA genotype. Conclusion GG genotype is associated with increased susceptibility to gastric cancer in high risk area of Xi＇an.