肿瘤坏死因子-α-308基因多态性与广东汉族人颅内动脉瘤的相关性研究

Relationship between tumor necrosis factor-α-308 gene polymorphism and intracranial aneurysm in Chinese Guangdong Han population

目的探讨广东汉族人群肿瘤坏死因子(TNF)-α-308基因多态性与颅内动脉瘤(IA)的相关性。方法采用聚合酶链-限制性片段长度多态性(PCR-RFLP)方法检测115例IA患者与100名健康对照者TNF-α-308基因多态性,并对两组人群中该基因的基因型频率进行比较。结果在IA患者中,G/G基因型有87例(75.7%),G/A基因型有28例(24.3%);在对照组中,G/G基因型有88名(88.0%),G/A基因型有12例(12.0%)。TNF-α-308基因型频率在病例组与正常对照组差异有统计学意义(P<0.05)。关联分析显示A等位基因携带者患IA的风险较正常对照者升高2.36倍(OR=2.36,95%CI=1.13～4.94)。结论TNF-α-308基因多态性与中国广东汉族人群IA的发生具有相关性,该基因可能是广东汉族人IA的易感基因之一。

Objective To investigate an potential association between tumor necrosis factor-α-308 gene polymorphism and intracranial aneurysm （IA） in Cbinese Guangdong Han population, Methods Tumor necrosis factor-α-308 gene polymorphism were examined in 115 patients with IA and 100 normal controls using PCR-RFLP technique. Results Only TNF-α gene-308G/G and G/A genotypes were detected in the study population. In the patients, G/G genotype and G/A were detected in 87 （75.7%） and 24 （24.3%） cases, respectively, In normal controls, G/G genotype and G/A were detected in 88 （88.0%） and 12 （ 12.0% ） eases, respectively. The difference in the genotype frequencies between the patients and controls was significant （ P 〈 0. 05 ）. Patients bearing homozygotes for A allele had a higher risk for IA compared with controls （ OR = 2.36, 95 % Confidence interval = 1.13 - 4. 94 ）. Conclusions The TNF-α-308 gene polymorphism is significantly associated with IA in Guangdong Han population, TNF-a gene may be a susceptible gene for IA in Chinese Guangdong Han population.