双色茉莉茎段离体培养再生植株的研究

In Vitro Plant Regeneration From Brunfelsia latifolia Stem

以双色茉莉茎段为外植体,探讨外植体取材季节和部位、基本培养基种类、植物生长调节剂组合、活性炭等对茎段器官发生和植株再生的影响。结果表明:在4月和10月取材最好,其诱导率分别达到了92.78%和84%,污染率最低;1/2MS+0.01 mg/L NAA+3.0 mg/L BA+2.0 mg/L KT培养基最适合不定芽的诱导分化,不定芽分化率达到了88.34%;在继代增殖培养中,继代次数最好在6代以内,否则增殖率下降,MS+0.5 mg/L IBA+3.0 mg/L BA+0.2%活性炭培养基最适合不定芽的增殖(5.92)和大于2 cm芽的分化(47.16%)。不定芽可在1/2MS培养基中有效伸长,适宜的生根培养基为1/2MS+1.0 mg/L IBA+0.2 mg/L BA,生根率达到100%。

Study was made on effect of stem collection season and part, basic medium, regulator combinations and activated carbon on in vitro plant regeneration of Brunfelsia latifolia. The result showed that shoot induction rate could reach 92.78% and 84% respectively with the lowest pollution rate by the stems collected for explant in April and October. 1/2MS ＋ 0.01mg/L NAA ＋ 3.0mg/L BA ＋ 2.0mg/L KT was the optimum medium for adventitious bud induction, topping 88.34% of differentiation ratio. The optimum medium for bud proliferation and differentiation was MS＋0.5mg/L IBA＋3.0mg/L BA＋0.2% ＋ 0.2% activated carbon and subculture generation was six. Adventitious buds could effectively grow in 1/2MS medium. Rooting rate topped 100% with 1/2MS ＋ lmg/L IBA＋ 0.2mg/L BA.