利用辣木茎段建立植株再生体系的研究

Establishment of Regeneration System in Vitro for Moringa oleifera with Stem

选择印度改良辣木的幼苗茎段,经外植体消毒后,进行了不定芽初代诱导、继代培养、生根诱导和生根苗移植试验,结果表明:最适不定芽初代诱导培养基为MS+6BA1mg/L+卡拉胶5g/L+糖30g/L,继代培养基为MS+6BA0.4mg/L+NAA0.2mg/L+卡拉胶5g/L+糖30g/L,生根培养基为1/2MS+IBA0.4mg/L+NAA0.2mg/L+卡拉胶7g/L+糖20g/L,最适的生根瓶苗移栽基质为黄心土40%+泥炭60%,并对微繁体系建立过程中继代苗的玻璃化、生根苗黄化及愈伤头过大、移栽过程中的管理等问题进行了讨论。

Experiments were conducted on establishment of regeneration system in vitro for Moringa oleifera from selecting seedling stem as explant, and after being sterilized for adventitious bud induction, subculture, rooting induction and transplanting of seedling. Results showed that culture medium MS containing 6-BA 1.0mg/L ＋ karagum 5g/L ＋ sucrose 30g/L was the best for adventitious bud induction, the optimal subculture medium was MS ＋ 6-BA 0Amg/L ＋ NAA 0.2mg/L ＋ karagum 5g/L ＋ sucrose 30g/L and the optimal rooting culture medium was 1/2MS ＋ IBA 0.4mg/L ＋ NAA 0.2mg/L ＋ karagum 7g/L ＋ sucrose 20g/L. For the rooting plantlets, the optimal transplanting medium was yellow earth 40% ＋ tuff 60%. Discussions were made on subculture vitrification, seeding etiolate, overgrowth of callus, and method of seedling transplantation.