生活垃圾堆肥草坪中蚯蚓对植物生长和抗氧化酶系的影响

Effects of earthworms on growth and antioxidant enzyme system of turfgrasses grown in municipal solid waste compost

通过在添加蚯蚓的生活垃圾堆肥基质上培植黑麦草、高羊茅和早熟禾,测定了草坪植物生物量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,研究了蚯蚓活动对3种草坪植物生长和抗氧化酶系的影响。结果表明:蚯蚓活动使黑麦草、高羊茅的地上生物量分别增加了3.53倍和12.17倍,地下生物量分别增加127.45%和148.94%,而早熟禾地上生物量减少42.37%。放入蚯蚓后,黑麦草和高羊茅的MDA含量分别减少了51.30%和87.52%,早熟禾增加了16.74倍。黑麦草、高羊茅和早熟禾的SOD和POD活性在放入蚯蚓后,分别有所下降,SOD活性分别下降了64.93%,13.38%和83.56%,POD活性分别下降41.15%,54.41%和34.09%。黑麦草CAT活性提高了5.61%,而高羊茅和早熟禾分别下降11.73%和33.23%。可见,生活垃圾堆肥草坪中蚯蚓活动对植物生长及抗氧化酶系的影响因植物种类而异。

To investigate impact of earthworms on growth and antioxidant enzyme system of three turfgrasses, Lolium perenne L., Festuca arundinacea L. and Poa pratensis L., we studied the effects of earthworms on biomass, malonaldehyde （MDA） content, as well as the activities of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） of the turfgrasses grown in municipal solid waste （MSW） compost medium by adding earthworms into the MSW medium. Our results showed that addition of earthworms into the MSW medium increased the aboveground biomass of L. perenne and F. arundinacea by 3.53 and 12.17-fold, respectively. The same treatments enhanced the underground biomass of L. perenne and F. arundinacea by 127.45% and 148.94% respectively. By contrast, the aboveground biomass of P. pratensis was decreased by 42.37% when the earthworms were introduced into the MSW medium. MDA content in L. perenne and F. arundinacea was reduced by 51.30% and 87.52% with addition of earthworms into the MSW medium, respectively. An increase in MDA content in P. pratensis by 16. 74 times was found by addition of earthworms into MSW medium.Furthermore, the presence of earthworms in the MSW medium had significant effects on antioxidant enzymes of the turfgrasses. Activities of SOD in L. perenne, F. arundinacea and P. pratensis were decreased by 64.93%, 13.38% and 83.56%, respectively. Activities of POD L. perenne, F. arundinacea and P. pratensis were increased by 41. 15%, 54.41% and 34. 09%, respectively. Activities of CAT in L. perenne were increased by 5. 61% with inclusion of earthworms in the MSW medium, whereas the same treatments led to decreases in CAT activities in F. arundinacea and P. pratensis by 11.73% and 33.23%, respectively. In conclusion, the present study demonstrates that earthworms in medium can markedly affect growth and the activities of antioxidant enzymes of turfgrasses, and that the effect is species-specific.