摘要
研究了umu试验菌株(Salmonellatyphimuri muTA1535/pSK1002)的菌种在普通冰箱中保存时其生长曲线和活性的变化,菌种经活化后可以得到稳定的活化生长曲线以及比较稳定的4-NQO活性曲线。通过菌种活化,使得umu试验菌种在普通冰箱中保存就能具有很好的活性和稳定性,极大地降低了保存难度,简化了菌种保存操作步骤,增加了试验的可重复性,有利于umu试验在实际环境样品检测中的应用。
Series of experiments were performed to study growth profiles and activity change of the umu test strain (Salmonella typhirnurimu TA1535/pSK1002) preserved in the freezer of an ordinary refrigerator (--20 ℃). Activation of the bacterial culture prior to the growth tests significantly reduced the effects of storage period and growing time on the growth profile and β-Galactosidase activity. The simple preservation procedure employing a general refrigerator and pre-activation before the test is attractive for use with the umu tests of actual environment samples.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2008年第10期48-50,54,共4页
Environmental Pollution & Control
基金
河南省教育厅科技攻关项目(No.2008A610002)
河南师范大学应用科学与技术研究基金资助项目(No.2007Y05)