umu试验菌种保存条件优化研究

Optimization of the preservation condition of umu test strain

研究了umu试验菌株(Salmonellatyphimuri muTA1535/pSK1002)的菌种在普通冰箱中保存时其生长曲线和活性的变化,菌种经活化后可以得到稳定的活化生长曲线以及比较稳定的4-NQO活性曲线。通过菌种活化,使得umu试验菌种在普通冰箱中保存就能具有很好的活性和稳定性,极大地降低了保存难度,简化了菌种保存操作步骤,增加了试验的可重复性,有利于umu试验在实际环境样品检测中的应用。

Series of experiments were performed to study growth profiles and activity change of the umu test strain （Salmonella typhirnurimu TA1535/pSK1002） preserved in the freezer of an ordinary refrigerator （--20 ℃）. Activation of the bacterial culture prior to the growth tests significantly reduced the effects of storage period and growing time on the growth profile and β-Galactosidase activity. The simple preservation procedure employing a general refrigerator and pre-activation before the test is attractive for use with the umu tests of actual environment samples.