污灌蔬菜中有机污染物致小鼠的遗传损伤与氧化损伤

RESEARCH ON GENETIC TOXICITY AND OXIDIZED DAMAGE OF ORGANIC EXTRACTION OF VEGETABLES FROM DIRTY IRRIGATION AREA ON MICE

[目的]研究污灌农产品有机提取物对小鼠的遗传损伤及其氧化损伤机制。[方法]选取某污灌农田产出的大白菜为研究对象,地下水灌溉的大白菜为对照,采用超声振荡法提取白菜中的有机污染物,对小鼠灌胃染毒后,进行单细胞凝胶电泳实验和微核试验及总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)活力和丙二醛(MDA)含量的测定。[结果]与试剂对照组比较,对照区高剂量组的拖尾率增高、T-SOD活力降低(P﹤0.05);污灌区拖尾率和拖尾长度和微核率均增高,且高于对照区(P﹤0.05);污灌区T-SOD和GSH-PX活力均降低(P﹤0.01和P﹤0.05),且低于对照区(P﹤0.05)。[结论]该污灌区白菜中有机提取物中含有致小鼠遗传毒性及氧化损伤的有机污染物,其毒性强于对照区。

[ Objective ] To study the genotoxicity and oxidized damage of organ extraction of agricultural products from irrigation area on mice. [Methods] Organ extractions were obtained from pollution area and control area. The way to extract organic pollutant was using sonic oscillation method. And aider using narcotics to the mice through lavage, a single-cell gel electrophoresis experiments and a mieronucleus test and T-SOD, GSH-PX, MDA were taken. [ Results] Compared with the reagent control group, the trailing length and T-SOD activity increased in the control group （P 〈 0.05） ; compared with the reagent control group and the control area. the trailing length, the trailing rate and mieronuelear rates increased in the irrigation area （P 〈 0.05）; T-SOD and GSH-PX activity decreased in the irrigation area （P 〈 0.05）. [ Conclusion] Organ extraction from irrigation area could cause genotoxicity and oxidized damage in mice, but the toxicity of organic matter in irrigation area is stronger than control area.