核因子-κBp50和P16^(INK4A)在尖锐湿疣皮损组织中的表达及意义

EXPRESSION OF NF-ΚB P50 AND P16^(INK4A) IN CONDYLOMA ACUMINATUM AND ITS SIGNIFICANCE

[目的]探讨核因子-κBp50(NF-κBp50)和P16INK4A在感染HPV6/11、HPV16/18型尖锐湿疣(condylo-mata acuminate CA)组织中的表达及其意义。[方法]采用荧光定量PCR法检测确定CA组织HPV6/11阳性同时HPV16/18阴性26例;HPV16/18阳性同时HPV6/11阴性21例;免疫组织化学Envision二步法检测26例低危型CA、21例高危型CA和18例正常包皮组织中NF-κBp50和P16INK4A的表达。[结果]NF-κBp50阳性表达主要定位于细胞浆,部分细胞核也表达,NF-κBp50在正常包皮、低危型、高危型.CA三组间阳性表达强度比较有统计学意义(χ2=26.739,P﹤0.05),P16INK4A阳性表达主要定位于细胞浆,P16INK4A在正常包皮、低危型、高危型CA三组间比较表达程度无差异(χ2=5.860,P﹥0.05)。[结论]NF-κBp50的表达增高可能在CA细胞增殖中起一定作用。

[Objective] To investigate the expression of NF-kB and P16^INK4A in condyloma acuminata （CA） with HPV6/ 11 positive and with HPV16/18 positive. [Methods] Twenty six patients with CA were diagnosed by fluorescent PCR, which HPV 6/11 was positive and HPV 16/18 was negative. Twenty six patients with CA were diagnosed by fluorescent PCR, which HPV 6/11 was positive and HPV 6/11 was negative. Expression distribution of NF-kBp50 ＆ P16^INK4A were detected by immuno- histochemistrv technique （envision）. which was in lesions of twenty one cases of CA with HPV6/11 twenty six eases of CA with HPV16/18 and fifteen ease normal human skins （foreskins）. [ Results] NF-kBp50 expression mainly localized in the cytoplasra, some expression localized in the nucleus, NF-kBp50 in normal foreskin, CA with HPV6/11 positive, CA with HPV16/18 positive groups, which expressions had differences （x^2 = 26.739, P 〈 0.05）. P16^INK4A positive expression mainly localized in the cytoplasm, P16^INK4A in the normal foreskin, low-risk type, high-risk CA groups, which expressions were no differences in degrees （x^2 = 5.860, P 〉 0.05）. [Conclusion] Increased NF-kBp50 expression in the CA cell proliferation may play a role.