超声辅助提取银杏外种皮多糖工艺优化研究

Study on the Technology Optimization of Extracting Polysaccharide from the Episperm of Ginkgo biloba by Ultrasonic-assisted Extraction

[目的]研究银杏外种皮中多糖的超声波辅助水提最优工艺。[方法]采用苯酚-硫酸法测定多糖含量,以多糖得率为评价指标,利用单因素试验和L9(34)正交试验考察超声波作用时间、超声功率、热水浸提时间和水浸提温度对银杏外种皮多糖提取的影响。[结果]各因素影响程度依次为:水浸提温度>水浸提时间>超声功率>超声时间,得到最优组合为:超声时间5 min,超声功率162 W,水浸提时间120 min,浸提温度100℃,在此工艺条件下多糖的提取率达16.529%。[结论]超声波辅助水浸提银杏外种皮多糖能提高提取效率,是一种节能、省时的好方法。

[ Objective ] The research aimed to study the optimum technology of extracting polysaccharide from the episperm of Ginkgo biloba by supersonic-assisted water extraction. [ Method] The content of the polysaeeharide was determined by the method of phenol-sulfuric acid. With the yield of polysaccharide as the evaluaton index, the effects of ultrasonic time, ultrasonic power,extracting time with hot water and water extraction temperature on the yild of polysaeeharide were investigated by using single factor experiment and L9 （3^4） orthogonal experiment. [ Result ] The influencing degree of each factor was as follows： water extraction temperature 〉 water extraction time 〉 ultrasonic power 〉 ultrasonic time. The optimum eombination was obtained as： ultrasonic time of 5 min, ultrasonic power of 162 W, water extraction time of 120 min and extraction temperature of 100 ℃. Under these technology conditions, the extraction ratio of polysaeeharide reached 16.529%. [Conclusion] Extracting polysaccharide from the episperm of G. biloba by ultrasonic-assisted extraction could enhance the extraction efficieney. It is a time-saved and energy-saved method.