生姜芽的组培快繁

Tissue Culture Propagation on Bud of Ginger

[目的]寻找生姜芽组培快繁的最佳方案。[方法]以MS为基础培养基,设计生姜芽组培快繁的两条途径,选择生姜芽组培快繁的最佳培养基配方。[结果]结果表明,茎尖→芽最适培养基为:改良MS+6-BA 3.0 mg/L+NAA 0.1 mg/L;茎尖→愈伤组织→芽,茎尖诱导愈伤组织的最适培养基为:改良MS+2,4-D 2.0 mg/L+KT 1.0 mg/L,愈伤组织诱导芽分化的最适培养基为:改良MS+6-BA 2.0 mg/L+NAA 0.5 mg/L。采用超净工作台上用75.0%酒精30 s+10.0%NaClO 15 min+0.1%HgCl210 min+50 ug/L青霉素与台外消毒相结合对外植体进行表面消毒,去污效果最佳。[结论]对愈伤组织进行切割并继代培养后再诱导芽分化,可获得较大的增殖倍数。

[ Objective ]The study was to search the optimal scheme of tissue culture propagation on bud of ginger. [ Method]With MS as basic medium, two approachs of propagation were desired in culture experiment to choose the optimal mediums of tissue culture propagation on bud of ginger. [Result] The results indicated that the optimal mediums was MS ＋ 6-BA 3.0 mg/L ＋ NAA 0.1 mg/L of stem tip→bud, MS ＋ 2,4-D 2.0 mg/L＋ KT 1.0 mg/L for bud callus induction and MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.5 mg/L for callus induce buds of stem tip→callus→bud, respectively. The decontamination effect was best through the surface disinfection on explant by combination of 75.0% alcohol 30 s ＋ 10.0% NaClO 15 min ＋ 0.1% HgCl2 10 min ＋ 50 ug/L penicillin and out-table disinfection on super-clean worktable. [ Conclusion] The big multiplication multiple will be obtained by induction bud differentiation after cutting callus and then subculture.