肠黏膜血流改变在创伤性脑损伤大鼠肠黏膜屏障应激性变化中的作用

Influence of the Change of Intestinal Blood Flow on the Intestinal Mucosa in Rats with Serious Traumatic Brain Injury

目的探讨肠黏膜血流改变在创伤性脑损伤大鼠肠黏膜屏障应激性变化机制中的作用。方法雄性Wistar大鼠64只,随机分为创伤性脑损伤组和假手术组,2组大鼠分别按术后6、12、24和48小时时相点分为4个亚组(n=8),测定肠黏膜血流量,光镜和透射电镜下观察肠黏膜组织形态学变化。结果创伤性脑损伤组各时相点的肠黏膜血流量均低于假手术组(P<0.05);光镜下创伤性脑损伤组肠黏膜上皮细胞受损,电镜下可见肠黏膜细胞间紧密连接较假手术组增宽。结论创伤性脑损伤后早期肠黏膜屏障即已受损,而肠黏膜血流量的下降是导致这一病理生理变化的重要因素之一。

Objective To investigate the influence of the change of intestinal blood flow on the damage of intestinal mucosa in rats with serious traumatic brain injury （TBI）. Methods Sixty-four healthy male Wistar rats were randomized into two groups ： TBI model group （ n = 32 ）, in which TBI rat models were established by Feeney＇s method ; and control group （ n = 32）, in which rats received sham operation. Each group was divided into four subgroups （ n = 8 each） according to the postoperative time points （6, 12, 24, and 48 hours after acute brain trauma）. The ileum tissue was carefully examined by light microscopy and electron microscopy. The intestinal blood flow was detected by laser Doppler flowmetry. Results The intestinal blood flow in TBI group was signif- icantly lower than that in control group （P 〈 0.05 ）. The intestinal mucosa was damaged and the intercellular space of intestinal mucosal epithelial cells was significantly larger in TBI group than in control group. Conclusion The decrease of intestinal blood flow play important roles in the stress-related damage of intestinal mucosa barrier in TBI rats.