滞育家蝇幼虫糖的变化

The change of carbohydrate of Musca domestica larva in the diapause

目的观察家蝇幼虫滞育前后血淋巴中总糖、海藻糖、糖原的变化,探讨家蝇幼虫滞育发生的生化机制。方法低温诱导家蝇幼虫滞育,用蒽酮比色法测定滞育10d、15d、20d组及非滞育组血淋巴总糖、海藻糖、糖原的含量。结果滞育组与非滞育组比较,(1)家蝇幼虫血淋巴总糖含量滞育10d组显著升高,15d组、20d组则降低。(2)血淋巴海藻糖含量滞育10d组、20d组明显升高,15d组差异无显著性。(3)血淋巴糖原含量滞育10d组、15d组降低,20d组则升高。结论家蝇滞育幼虫的糖醇积累型属于海藻糖积累型。

Objective To observe the changes of carbohydrate in the hemlymph of Musca domestica larva before and after in the diapause. To explore the biochemistry mechanism in the diapause of Musca domestica larva. Methods The diapause of the Musca domestica larva was inducted by low temperature. The contents of total carbohydrate , trehalose and glycogen in the hemlymph of Musca domestica larva in the diapause 10d, 15d, 20d groups and the nondiapause group were determimined by anthrone chromatometry, respectively. Results Compared with the diapause groups＂ and the nondiapause group, the content of the hemlymph total carbohydrate was increased significantly in the diapause 10d group, but were decreased in the 15d and 20d groups ; the contents of the hemlymph trehalose were increased significantly in the diapause 10d and 20d groups, and there were not differences in the diapause 15d group; the contents of the hemlymph glycogen were decreased in the diapanse 10d and 15d groups , but was increased significantly in the 20d group. Conclusion The accumulation model of carbohydrate alcohol in the diapause larva of the Musca domestica was belonged to the model of trehalose.