米诺环素与牛血清白蛋白相互作用的研究

Interaction of minocycline and bovine serum albumin

目的:研究人体生理条件下米诺环素与牛血清白蛋白(BSA)的相互作用机制。方法:利用荧光光谱法,并以Stern-Volmer方程确定药物与蛋白的作用类型。结果:根据不同温度下米诺环素对BSA的荧光猝灭作用,证明两者间为单一的动态猝灭过程,根据Stern-Volmer方程求出了米诺环素与牛血清白蛋白(BSA)的猝灭常数,并根据F儯rster能量转移理论确定了生理条件下药物与蛋白的结合距离为3.03 nm。结论:在人体生理条件下米诺环素对牛血清白蛋白具有荧光猝灭作用且为动态猝灭过程。同步荧光技术确定米诺环素对BSA构象有一定的影响。

Objective：To study the interaction of minocycline and bovine serum albumin （BSA） in physiological solution. Method： Using fluorescence spectroscopy and based on the equation of Stern-Volmer, the type of interaction between drug and BSA was determined. Results：According to the quenching effect of minocycline on the fluorescence intensity of BSA at different temperatures, the quenching mechanism was confirmed to be a dynamic one. Quenching constant was obtained according to equation of Stern-Volmer and the distance between minocycline and BSA was 3.03 nm determined according to the Farster theory of non-radiation energy transfer. Conclusion： It is proved that the interaction of minocycline and BSA is a dynamic quenching in physiological solution. The conformational change in BSA due to the minocycline addition is confirmed by synchronous fluofimetry.