期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RT-PCR检测门静脉血CK20mRNA对诊断结肠癌肝脏微转移的意义 被引量：2

原文传递
导出
摘要 目的探讨结肠癌患者门静脉血CK20mRNA的检测对肝脏微转移的诊断价值。方法采用RT-PCR技术检测31例结肠癌患者门静脉血中CK20mRNA。结果全组检出CK20mRNA阳性率为25.81%,随访时间平均(18.5±10.6)个月(6～32个月)。1例术中发现肝转移且门静脉血CK20为阳性;术时未见肝脏转移而门静脉血CK20阳性的7例中3例术后(6～15个月)出现肝转移(42.85%);术时未见肝转移且门静脉血CK20阴性的23例,2例出现肝转移(8.69%)。结论结肠癌术中门静脉血CK20mRNA的检测对于患者的预后和指导治疗有参考价值。
作者 黄志恒 刘民生 王平 王成友
机构地区 广东省深圳市第二人民医院器官移植科
出处 《中国普通外科杂志》 CAS CSCD 2008年第10期1030-1032,共3页 China Journal of General Surgery
基金 深圳市科技局资助项目(200603064)
关键词 结肠肿瘤 CK20MRNA 微转移 实时荧光定量RT-PCR
分类号 R735.3 [医药卫生—肿瘤]
  • 相关文献

参考文献7

  • 1Wyld DK, Selby P, Perren TJ, et al. Detection of eolorectal cancer cells in peripheral blood by reverse-transcfiptase polymerase chain reaction for cytokeratin20 [ J ]. Int J Cancer, 1998,79(3) :288 -293.
  • 2Chen YH, Gao WH, Zhou TL, et al. Department of bone marrow micrometastasis [ J ]. Hybridoma, 1999, 18 (4) :464 -466.
  • 3Ghossein RA, Rosai J. Polymerase chain reaction in the detection of micrometastasis and circulating tumor cells [ J ]. Cancer, 1996,78(1) :10-16.
  • 4刘民生,黄志恒,杨锋,郑凯,徐敏,何丽娟.大肠癌患者门静脉血CK20mRNA检测的临床意义[J].实用诊断与治疗杂志,2007,21(1):1-2. 被引量：6
  • 5蔡成机.对胃、结直肠癌肝转移几个临床问题的思考[J].中国普通外科杂志,2005,14(10):721-722. 被引量：23
  • 6Deans G T, Parks T G, Rowlands B J, et al. Prognostic factors in colorectal cancer [ J ]. Br J Surg, 1992,79 (5) :608 -613.
  • 7段明松,苏加庆,周增祥.结直肠癌转移相关基因的研究进展[J].中国普通外科杂志,2007,16(9):890-891. 被引量：4

二级参考文献23

  • 1周坤,葛海燕.腹腔镜结直肠癌根治术对肿瘤细胞微转移影响的研究[J].中国普通外科杂志,2005,14(12):896-899. 被引量：5
  • 2李伟伟,杨玉秀.粪便分子学检测在结肠癌筛查中应用[J].实用诊断与治疗杂志,2006,20(4):272-274. 被引量：7
  • 3沈岚,谭洁.熊果酸诱导结肠癌HT-29细胞凋亡与凋亡相关基因的关系[J].实用诊断与治疗杂志,2006,20(11):784-786. 被引量：8
  • 4Park S K,Kim H S,Hong E K,et al.Expression of cytokeratins 7 and 20 in primary carcinomas of the stomach and colorectum and their value in the differential diagnosis of metastatic carcinomas to the ovary[J].Hum Pathol,2002,33(10):1078-1085.
  • 5Keisuk K,Hayan N,Naoki H,et al.Quantitative detective of micrometastases in the lymph nodes of gastric cancer patients with real-time RT-PCR,A comparative study with immunohistochemistry[J].Int J Cancer,2003,105(2):136-143.
  • 6Zshida H,Murata N,Tada M,et al.Determining the levels matrix metalloproteinase-9 in portal and peripheral blood is useful for predicting liver metastasis of colorectal cancer[J].Jpn J Clin Oncol,2003,33 (4):186-191.
  • 7Zucker S,Lysik RM,Dimassimo BI,et al.Plasma assay of gelatinase B:tissue inhibitor of metalloproteina complexes in cancer[J].Cancer,1995,76(4):700 -708.
  • 8Lee JC,Chow NM,Wang ST,et al.Prognostic value of vascular endothelial growth factor expression in colorectal cancer patients[J].Eur J Cancer,2000,36 (6):748-753.
  • 9Yamaguchi A,Nakagawara G,Kurosaka Y,et al.p53 immunoreaction in endoscopic biopsy specimens of colorectal cancer,and its prognostic significance[J].Br J Cancer,1993,68(2):399 -402.
  • 10Nystrom Lahti M,Wu Y,Moisio AL.DNA mismatch repair gene mutation in 55 kindreds with verified or putative hereditary non-polyposis colorectal cancer[J].Hum Mol Genet,1996,5(6):763 -769.

共引文献30

同被引文献22

  • 1耿强,王水,范萍,刘晓安,郑伟.乳腺癌前哨淋巴结和骨髓和外周血微小转移的联合检测[J].肿瘤防治杂志,2005,12(2):85-88. 被引量：8
  • 2杨华伟,刘剑仑,曹骥,杨南武,苏建家.乳腺癌患者外周血SBEM-mRNA和CD44V6-mRNA的检测及其临床意义[J].中国肿瘤临床,2005,32(7):382-385. 被引量：9
  • 3佘明金,秦凤展,吴穷,郑荣生,汪子书.GalNAcT和CK19mRNA表达与乳腺癌骨髓微转移的相关性[J].蚌埠医学院学报,2005,30(6):490-492. 被引量：3
  • 4李金贵（综述）,胡自力（审校）.存活素基因与肿瘤的研究现状[J].国际检验医学杂志,2007,28(5):462-464. 被引量：9
  • 5Fisher B. Personal contributions to progress in breast cancer research and treatment[J]. Semin Oncol, 1996,23 (4) :414-427.
  • 6Gaubin M, Autiero M, Basmaciogullari S,et al. Potent inhibition of CD4/TCR-mediated T cell apoptosis by a CD4-binding glycoprotein secreted from breast tumor and seminal vesicle cells[ J]. J Immunol, 1999,162 ( 5 ) : 2631-2638.
  • 7Mitas M, Mikhitarian K, Walter C, et al. Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multi-gene marker panel [ J ]. Int J Cancer,2001,93 ( 2 ) : 162-171.
  • 8Ntolia M, Stathopoulou A, Igatiadis M, et al. Detection of mammaglobin a-mRNA-positive circulating tumor cells in periphetal blood of patients with operable breast cancer with nest RT-PCR [J]. Clin Biochem,2006,39(9):879-887.
  • 9Stoupoulou A, Mavioudis D, Perraki M, et al. Molecular detection of cancer cells in the peripheral blood of patients with breast cancer. Comparison of CK-19, CEA and maspin as detection markers[J]. Antieancer Res,2003,23(2) :1883-1890.
  • 10Kwon S, Kang SH, Ro J,et al. The melanoma antigen gene as a surveillance marker for the detection of circulating tumor cells in patients with breast carcinoma [J]. Cancer, 2005,104 ( 2 ) : 251- 256.

引证文献2

中国普通外科杂志
2008年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部