氩激光诱导小鼠脉络膜新生血管形成

Choroidal Neovascularization Mouse Model Induced by Argon Laser

目的探讨应用氩激光诱导C57BL/6J小鼠脉络膜新生血管(choroidal neovascularization,CNV)模型的可行性。方法雄性C57BL/6J小鼠28只,用数字表法随机等分为7组,每组4只。用氩激光光凝小鼠双眼视网膜,于光凝后2h、3d、7d、10d、14d、28d和60d观察眼底情况,并行眼底荧光血管造影(fundus fluorescein angiography,FFA),于检查后立即处死,摘取眼球行光学显微镜(lightmicroscopy,LM)检查。结果FFA和LM检查均证实,光凝后7d CNV开始形成,10d渐增多,28d到高峰并能保持稳定,60d有所减少。结论氩激光能成功诱导C57BL/6J小鼠CNV模型,成模所需时间短,成模率高,持续时间较长,操作方便,是一种比较理想的CNV动物模型复制方法。

Objective To evaluate the feasibility of inducing choroidal neovascularization （CNV） in C57BL/6J mice by argon laser. Methods Twenty-eight C57BL/6J mice were divided into 7 groups. In each group, the mice receieved retinal photocoagulation by argon laser in both eyes, at 2 hours, 3, 7, 10, 14, 28 and 60 days after photocoagulation, the formation and nature process of CNV were observed by fundus fluorescein angiography（ FFA）, and lightmicroscopy（LM）. Results FFA and LM demonstrated that CNV began to form at the seventh day after photocoagulation, increased at the tenth day, reached the peak at the twenty-eighth day and kept stable until sixty day. Conclusion Argon Laser can successfully induce CNV in C57BL/6J mice, and it is an ideal animal model for further study.