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HDAC7基因参与兔高脂间歇低氧诱发动脉粥样硬化 被引量：2

HDAC7 gene involved in atherosclerosis induced by intermittent hypoxia and hyperlipemia in rabbits
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摘要 目的研究高脂间歇低氧诱发动脉粥样硬化形成的重要基因。方法提取高脂组和高脂间歇低氧组动脉粥样硬化兔模型肝组织总RNA,用抑制性消减杂交技术,构建差异表达的cDNA消减文库,用反向Northern blot法鉴定存在差异表达的基因,对插入片段测序后进行blast的同源性搜索未知基因,定量RT-PCR验证。结果获得的组蛋白脱乙酰基酶7(HDAC7)为未知功能新基因,在两组肝脏组织中表达存在显著差异。结论HDAC7可能是高脂间歇低氧诱发动脉粥样硬化发生中发挥重要作用的新基因。 Objective To investigate gene expression patterns of atherosclerosis （As） induced by intermittent hypoxia and hyperlipemia. Methods Isolate total mRNA of liver tissue from atherosclerosis rabbit model in hyperlipemia group and hyperlipemia with intermittent hypoxia group. Suppression subtractive hybridization was adopted to construct cDNA lib, and to identify differential expression by opposite Northern blot. The expression of histone deacetylase 7 was obviously different in two groups. Results MYL6 and HDAC7 were screened in cDNA lib. Conclusion The process of atherosclerosis induced by intermittent hypoxia is correlated to many genes. HDAC7 is a gene play new important role in AS.
作者 吴悦陶 刘瑞洪 黄谷香 张湘瑜 杨宇
机构地区 中南大学湘雅二医院老年病科
出处 《基础医学与临床》 CSCD 北大核心 2008年第10期1052-1056,共5页 Basic and Clinical Medicine
基金 湖南省科技厅资助项目(05SK3024)
关键词 高脂血症 间歇低氧 动脉粥样硬化 抑制性消减杂交 hyperlipemia intermittent hypoxia atherosclerosis suppression subtractive hybridization
分类号 R543.5 [医药卫生—心血管疾病]
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参考文献10

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共引文献4

同被引文献41

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基础医学与临床
2008年 第10期

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