摘要
目的观察次黄嘌呤、尿酸酶抑制剂尿囊毒酸对大鼠血清尿酸水平的影响,拟建立一种新型实验性大鼠痛风病理模型。方法采取合用次黄嘌呤和尿囊毒酸为造模药物,制备大鼠高尿酸血症动物模型,比较单用次黄嘌呤及合用尿囊毒酸对受试动物血清尿酸水平的影响,并对造模药物的量效、时效关系等因素进行了考察。结果次黄嘌呤与尿囊毒酸合用并以适当的剂量和给药方式,可使受试动物血清尿酸稳定持续增高(≥1000μmol/L),造成实验性高尿酸血症动物模型。结论实验证明单用次黄嘌呤不能升高大鼠的血尿酸水平,必须配合尿酸酶抑制剂尿囊毒酸,方可升高大鼠尿酸水平(P<0.01)。采用连续给药方式,动物血清尿酸水平可大幅持续升高。
Objective To observe the fluctuation of uric acid in rat serum with hypoxanthine, uricase inhibitor, and to establish a new method to replicate rat hyperuricemia model. Method Adopted the combination of hypoxanthine and uricase inhibitor to establish rat hyperuricemia model, and compared the uric acid contents in serum when using hypoxanthine singly with that of using the combination of hypoxanthine and allantoxanic acid. Simultaneously those factors of research methods have been evaluated, such as those proposed model-leading drug＇s dose-effect and time-effect relationship. Results The combination of hypoxanthine and uricase inhibitor could increase the content of uric acid in rat serum by means of appropriate dosage and method of administration （1000 μmol/L）. It could create the rat hyperuricemia model successfully. Conclusion The combination of hypoxanthine and allantoxanic acid could increase uric acid contents in rats rather than using hypoxanthine singly. Meanwhile, by repeatedly dosing regimen, uric acid contents in serum of rats could increase during rather longer time （P〈0.01）. So it has made some progress in replicating steady hyperuricemia model at present experiment.
出处
《实验动物与比较医学》
CAS
2008年第5期321-323,327,共4页
Laboratory Animal and Comparative Medicine
基金
2005年江苏省高校自然科学研究基金项目(No.05KJB310097)
