己酮可可碱对腹膜功能的影响及其机制研究

Effect and Mechanism of Pentoxifylline on Peritoneal Function

目的探讨己酮可可碱对腹膜功能的影响和可能机制。方法将雄性SD大鼠45只随机分成3组各15只,A组每日腹腔内注射0.9%氯化钠溶液20 mL,B组和C组每天腹腔注射4.25%腹膜透析液20 mL,并在第8,10,12天加入LPS75μg,C组在腹腔注射液中同时加入6 mg.kg-1己酮可可碱。5周后行2 h腹膜平衡实验测定腹膜功能,留取血液及腹膜透析液,ELISA法测定血管内皮生长因子(VEGF)浓度;同时处死大鼠,留取肠系膜组织,RT-PCR检测肠系膜组织VEGF mRNA表达水平。结果B、C组超滤量及2 h透出液葡萄糖浓度/0 h透析液葡萄糖浓度(D2/D0)比值均较A组明显减少(均P<0.05),B组较C组更明显;B、C组透析液尿素/血浆尿素浓度比值(D/Purea)较A组明显增加(P<0.05),B组较C组增加更明显;C组腹透液及血清中VEGF浓度,以及肠系膜VEGF mRNA表达较B组明显下降。血清及腹透液VEGF含量分别与D2/D0,D/Purea有较明显的相关性。结论己酮可可碱能有效改善腹膜功能,其机制可能与调节VEGF表达有关。

Objective To investigate the effect and possible mechanism of Pentoxifylline in treating peritoneal function of SD rats. Methods Rats were randomly divided into three groups： the control（ group A, given N.S. 20 mL daily） , model （ group B） and pentoxifylline treated （ group C ）, the two groups latter were i.p. 4.25 % high glucose peritoneal dialysis fluid 20 mL, added in LPS at 8 d, 10 d, 12 d. Besides. rats in group C were treated with 6 mg · kg^-1 pentoxifylline daily. Five weeks later, the function of peritoneum was evaluated by a 2-hour peritoneum equilibrium test （PET） and vascular endothelial growth factor （VEGF） in blood and dialysate was detected by ELISA method. Meanwhile, the expression of VEGF mRNA in mesenterium was detected by RT-PCR method. Results Glucose reabsorption（ D2/D0 ） and net uhrafiltration（UF） volume in group B and C were significantly lower than those in group A, especially for group B; Dialysate-to-plasma urea ratio（D/Purea） in group B and C was significantly higher than that in group A, group B was more obvious. In group C, the concentration of VEGF in blood and dialysate was decreased obviously and VEGF mRNA in mesentery was down regulated, compared with those in group B. There were significant correlations between the concentration of VEGF in blood and dialysate and D/Purea, D2/D0 , respectively. Conclusion Pentoxifylline is beneficial to peritoneal function, the mechanism of which may be associated with decreased expression of VEGF.