雌激素替代治疗对去势大鼠阴道组织雌激素受体表达的影响

Effect of Estrogen Replacement on Expression of Vagina Hormone Receptor in Oophorectomized Rats

目的研究雌激素对去势大鼠阴道组织ERα与ERβ表达的影响,探讨雌激素治疗老年性阴道炎的分子机制。方法将40只雌性SD大鼠随机分成对照组、假手术组、去势组和雌激素替代治疗组各10只。对照组为空白对照;假手术组去除卵巢周围与卵巢相同大小脂肪组织;去势组行双侧卵巢切除术;雌激素替代治疗组在去卵巢手术3周后行戊酸雌二醇800μg.kg-1.d-1替代治疗8周。免疫组织化学染色检测大鼠阴道组织ERα与ERβ表达情况。结果去势组大鼠ERα、ERβ表达显著低于对照组(均P<0.01),雌激素替代组ERα、ERβ表达上升,较去势组显著增强(均P<0.01)。结论雌激素替代治疗可导致雌激素受体表达上升,可能导致雌激素受体效应增强,从而提高阴道局部抵抗力。

Objective To investigate the effect of estrogen replacement on expression of vagina hormone receptor in oophorectomized rats and the molecular mechanism. Methods 40 SD Rats （12week-old） were randomly divided into four groups： contrast rats, sham operated rats, ovariectomized （OVX） rats, and ovariectomized rats with ERT （OVX ＋ EV）, 10 in each. The contrasts were normal rats, the sham operated rats were only cleared away fat tissues beside ovary with the same size of it. Meanwhile, both OVX and OVX ＋ EV ones were ovariectomized. 3 weeks after ovariectomy, rats were treated with estradiol valerate 800 ug · kg^-1· d^-1 for 8 weeks. The expression of estrogen receptor α and β in vagina tissues was detected by immunohistochemistry. Results The oophorectomized rats showed less ERα,ERβ expression than contrast ones in the vagina epithelial cell（ P 〈 0.01 ）, however, estrogen replacement therapy increased the expression （ P 〈 0.01 ）. Conclusion Estrogen replacement therapy enhances expression of estrogen receptor, which strengthening the response of receptors.