氢化可的松与辛伐他汀对高脂饮食大鼠动脉NF-κB与ICAM-1含量的影响

The Effect of Hydrocortisone and Simvastatin on Contents of NF-κB and ICAM-1 in Aorta of Hypercholesterolemic Rats

目的观察氢化可的松与辛伐他汀对高脂血症模型大鼠动脉核转录因子-κB(NF-κB)、血管细胞间粘附因子-1(ICAM-1)含量的影响,探讨其防治动脉粥样硬化(AS)的机制。方法将普通级SD大鼠随机分为空白组,高脂组,氢化可的松高、低剂量组和辛伐他汀组。空白组饲喂基础饲料,其他组饲喂造模饲料并给予相应药物,16周后采用放射免疫法检测血清中三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量;制作主动脉常规及免疫组化切片,观察内膜、肌层厚度及NF-κB、ICAM-1阳性细胞比例。结果氢化可的松高、低剂量组,辛伐他汀组主动脉内膜及肌层厚度明显薄于高脂组,厚于空白组,其NF-κB、ICAM-1阳性细胞率显著低于高脂组,高于空白组。结论氢化可的松、辛伐他汀有抗AS作用,其机制可能通过抑制NF-κB激活和降低ICAM-1分泌有关。

Objective To investigate the effect of hydrocortisone, simvastatin on contents of NF-kB and ICAM-1 in the aorta of hypercholesterolemie rats and its mechanism of preventing and treating atherosclerosis. Methods SD rats were divided into the blank group, high-fat diet group, hydrocortisone group, and simvastatin group. Rats in the blank group were fed with normal diet. Rats in other groups were fed with lipid-rich diet and drugs. 16 weeks later, contents of TG, TC, LDL in serum were detected by the radioimmunity method. The internal membrane, muscular layer and ratio of positive cells with NF-kB p65 and ICAM-1 were observed by immunohistochemistry technique. Results The aortic tunica intima and muscular layer in hydrocortisone group and simvastatin group were thicker than those in the high-fat diet-fed group, but thinner than those in the blank group. The ratio of the positive cell of NF-kB p65 and ICAM-1 in hydrocortisone group and simvastatin group were less than in high-fat diet group, and larger than those in the blank. Conclusion Both hydrocortisone and simvastatin have antiatherosclerosis effects, which could be attributed to inhibited activation of NF-KB and secretion of ICAM-1.