玫瑰组织培养快繁技术

The Rugosa Rose Tissue Culture Rapid Propagation Technology

以玫瑰新品种"17号"带芽的幼嫩茎段为外植体,采用添加不同生长激素的MS培养基,对其进行组织培养研究。结果表明:玫瑰茎段在MS+BA2.5mg/L+NAA0.2mg/L培养基上能很好地诱导芽形成,诱导率90%以上;在继代培养基MS+BA3mg/L+NAA0.1mg/L中增殖系数较高,为4;生根培基为1/2MS+NAA0.5mg/L;将生根瓶苗炼苗1周,移植至蛭石中,经过1～2个月的管理,即可定植于富含腐殖质的砂质壤土中。

This paper studies the effect of cytotins and auxins using in rapid propagation of rugosa rose tissue culture. The results show that MS media supplemented with BA2.5 mg/L ＋NAA 0.2 mg/L can induce shoots well, and the rate of inducement is more than 90%; in gerbera proliferation media, the effect of combining with BA3 mg/L ＋ NAA 0.1 mg/L is better than others, and on an average, 4 shoots are formed per original explant; the root media is 1/2MS＋NAA0.5 mg/L, the plantlets have been hardened for 1 week ,then transplanted them into the media which is consisted of vermiculite. One or two months later, the plantlets that have new roots are permanent planted in sandy loam full of humus.