羊栖菜提取物对食用菌竞争性杂菌生长的抑制作用

Inhibition effect of Sargassum fusiforme extracts on pathogenic fungi

采用生长速率法和孢子萌发法对羊栖菜提取物的抗真菌活性进行了筛选,结果表明:Rf0.47(10g.kg-1、1 g.kg-1)和Rf0.81(100 g.kg-1、1 g.kg-1)对圆弧青霉菌丝生长抑制率分别为35%、50%和85%、25%,羊栖菜乙醚提取物乙酸乙酯相的薄层分离带Rf0.30(100 g.kg-1)对绿色木霉及黑曲霉抑制率分别为75.67%、83.46%。相关系数分析显示:不同浓度的3个处理组存在极显著的正相关。双因素方差分析表明:分离带和浓度两因素对绿色木霉、圆弧青霉、黑曲霉菌丝生长抑制率的影响极显著。在3种质量浓度下,羊栖菜乙醇Rf0.47、Rf0.81、羊栖菜乙醚Rf0.00对圆弧青霉孢子萌发以及羊栖菜乙醇乙酸乙酯相、羊栖菜乙醚乙酸乙酯相、羊栖菜乙醚Rf0.30对黑曲霉孢子萌发均有显著的抑制作用。

Anti-fungal activities of the extracts from Sargassum fusiforme were tested by means of hypha growth rate and spore germination. The results indicated that the Rf0.47 at 10 g · kg^-1 ,1 g · kg^-1 and Rf0. 47 at 100 g · kg^-1 , 1 g · kg^-2 of the ethyl acetate phase on TLC from the S. fusiforme soaked in ethanol significantly inhibited Penicillium cyclopium growth. The inhibition rates of those components were 35%, 50%, 85 % and 25 %, respectively. The RsO. 30 at 100 g · kg^-1 of the same extract significantly inhibited Trichoderma viride and Aspergil lus niger V. Tiegh growth with inhibition rates of 75.67% and 83.46%, respectively. The correlation analysis showed that concentrations of the 3 treatment groups significantly correlated to the inhibitory effect. Two-factors analysis of variance showed that the separation and concentration affected significantly on T. viride, P. cyclopium, A. niger V. Tiegh mycelial growth inhibition. In different concentrations, Rf 0. 47, Rf 0.81 of Sargassum fusiforme soaked with ethanol, Rf 0. 00 of Sargassum fusiforme soaked with ether and ethanol ethyl acetate phase and ethyl acetate phase of Sargassum fusiforme ether, Rf 0. 30 of Sargassum fusiforme soaked with ether have the extremely significant inhitition on the germination of spores of Aspergillus niger V. Tiegh.