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动力蛋白轻链亚基LC8在人细胞内的亚细胞定位 被引量：1

Subcellular Localization Analysis of Dynein Light Chain LC8 in Human Cells
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摘要 动力蛋白是活跃于微管骨架上行使负向运输功能的分子马达.从人类B淋巴细胞cDNA文库中克隆得到动力蛋白轻链亚基的基因LC8,将该基因插入表达绿色荧光蛋白的载体pEGFP-C3,获得的重组DNA成功在两种人源细胞内表达.通过荧光实验对GFP-LC8亚细胞定位进行分析,确定GFP-LC8是典型细胞浆定位,主要分布在细胞外周区和微管组织中心附近.当用药物nocodazole处理细胞使微管解聚时,GFP-LC8的定位发生改变.表明融合蛋白可正确指示LC8的细胞定位,可用于进一步研究病毒感染后的胞内事件. Dynein is an effective molecular motor of the microtubule cytoskeleton, which is responsible for minus-end directed retrograde transport. In this study, a dynein light chain gene LC8 was amplified from a cDNA library of human B lymphocyte and inserted to a GFP-expressing vector pEGFP-C3. The recombinant DNA was then successfully expressed in human cells. By fluorescence assay, we confirmed a typical cytoplasmic localization of GFP-LCS. Cell peripheral localization and microtubule organizing center localization were the dominating distribution patterns of cytoplasmic GFP-LC8. A distinct subcellular localization of GFP-LC8 was detected in microtubule-depolymerizing agent treated cells. These results supported the further study of virus transport.
作者 苏旸 陈颜 吴晓静 拜晓勃 耿运琪 乔文涛 陈启民
机构地区 南开大学生命科学学院
出处 《南开大学学报（自然科学版）》 CAS CSCD 北大核心 2008年第5期37-42,共6页 Acta Scientiarum Naturalium Universitatis Nankaiensis
基金 国家自然科学基金(30770080) 天津市应用基础及前沿技术研究计划重点项目(08JCZDJC21000)
关键词 细胞骨架 微管 分子马达 动力蛋白 绿色荧光蛋白 LC8 cytoskeleton microtubules molecular motor dynein green fluorescent protein LC8
分类号 Q245 [生物学—细胞生物学]
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参考文献24

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二级参考文献16

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南开大学学报（自然科学版）
2008年 第5期

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