姜黄素固体分散制剂抗小鼠脂肪肝的实验研究

Experimental Study of Effects of Curcumin Solid Dispersion Preparation on Fatty Liver in Rats

目的观察姜黄素固体分散制剂在小鼠体内抗脂肪肝的作用。方法通过给予高脂饲料、乙醇灌胃、四氯化碳(CCl4)葵花籽油溶液皮下注射的复合方式建立小鼠脂肪肝模型,同时给予不同剂量的姜黄素。于试验第16天取血及肝组织,计算肝指数,进行血脂、肝脏内脂质检测及病理组织学检查。结果不同剂量的姜黄素均可不同程度地降低血清及肝匀浆中的甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)的含量,改善病理组织学检查结果,其中姜黄素300mg/kg组的作用最显著。与模型组相比,各试验组肝湿重和肝指数均有下降,无统计学意义。结论姜黄素具有一定防治脂肪肝的作用,并呈一定的剂量依赖性。

Objective To observe the preventing effect of Curcumin Solid Dispersion Preparation on fatty liver in rats. Methods Sixty mice were randomly divided into six groups, such as normal group, model group and three curcumin groups in different doses. The compound fatty liver models were induced by high-fat feeding, intragastric administration with the alcohol and subcutaneous injection with CCl4. On 16 d, all the mice were killed, and the contents of TG, CHO and LDL- C in the serum and the liver tissue were determined. The change of liver histology was observed. Results The contents of TG, CHO and LDL - C in the serum and the liver were decreased in the curcumin - treating groups compared with the model group. The liver histology and liver indexes of the curcumin- treating groups were better than those of the model group. Conclusion Curcumin can prevent fatty liver, and the effect may depend on its dose.