油茶根腐病病原菌分子鉴定及其生物学特性研究

Study on Biological Characteristics and Molecular Identification of Pathogens Causing Root Rot of Camellia oleifera

从油茶腐根中分离得到病原菌，通过对其形态特征的观察及该菌rDNA转录间隔区（ITS）序列的测定，与Genbank中经Blastn搜索获得的部分菌株构建分子系统发育树．基于形态特征和分子系统发育树，确定引起油茶根腐病的病原菌为镰刀属李瑟组层生镰刀菌（Fusarium proliferatum）．还对该菌的生物学特性进行了研究，发现其最适生长温度为25℃，最适pH值是6．0～7．0，最适碳源为可溶性淀粉，最适氮源为NH4Cl．

Pathogens from rotten root of Camellia oleifera were isolated and identified. Molecular phylogenetic trees of some pathogen strains were expatiated through morphological characteristics observation, rDNA transcription, sequence determination of the internal transcribed spacer （ITS） and by Blastn search in the Genbank. Based on morphological characteristics and molecular phylogenetic trees, it was concluded that the pathogens to cause root rot of Camellia oleifera was Fusarium moniliform. The study on biological properties of the isolated pathogen showed that the optimal temperature for the pathogen was 25℃, the optimal pH range for its growth was 6 -7, the best carbon source was soluble starch and the best nitrogen source was NH4Cl.