高效液相色谱-蒸发光散射测定血塞通注射液中三七皂苷R_1、人参皂苷Rg_1、Re及Rb_1的含量

Determination of Notoginsenoside R_1,Ginsenoside Rg_1,Re and Rb_1 in Xuesaitong Injection by HPLC-ELSD

目的：用HPLC—ELSD测定血塞通注射液中三七皂苷R1、人参皂苷Rg1、Re及Rb1的含量。方法：色谱柱填料为氨基键合硅胶，流动相为乙腈-水（80：20）；漂移管温度为90℃，载气流速为2.1L·min^-1。结果：三七皂苷R1、人参皂苷Rg1、Re及Rb1的线性范围和相关系数分别为：0．30—2．01μg（r=0．9991）、1．50～9．98μg（r=0．9997）、0．45～3．00μg（r=0．9992）及1，20—8．03μg（r=0．9996）；回收率（n=6）分别为103．1％（RSD=2．7％）、98．1％（RSD=2．3％）、102．8％（RSD=2．6％）及96．9％（RSD=2．5％）。结论：该方法简便、准确、分离效果好，无干扰，可用于血塞通注射液的质量控制。

Objective：To establish the quantitative method of Notoginsenoside R1, Ginsenoside Rg1 ,Re and Rb1 in Radix Notoginseng by HPLC-ELSD. Method： The column used was a Lichrospher NH2（4. 6 mm ×250mm） packed with a 5 μm stationary phase. The mobile phase consisted of acetonitrile-water（80： 20）. The temperature of drift tube was 90 ℃ and the nebulizer nitrogen flow rate was 2. 1 L ·min^- 1. Result： A good linearity was obtained with the correlation coefficients ranged from 0. 30 - 2. 01 μg （r = 0. 999 1 ）, 1.50 - 9. 98 μg（ r = 0. 999 7 ） ,0.45 - 3.00 μg（ r = 0. 999 2） and 1.20 - 8.03 μg（ r = 0. 999 6）, respectively. The average recoveries of the four components （ n = 6 ） were 103.1% （ RSD = 2. 7% ）, 98.1% （ RSD = 2. 3 % ）, 102. 8% （ RSD = 2.6% ） and 96. 9% （ RSD = 2. 5% ）, respectively. Conclusion： It was proved that the method with HPLC-ELSD was reliable, simple, well separated and precise, that could be used as principles of quantitative analysis.