HPLC法测定益肾灵颗粒中补骨脂素、异补骨脂素的含量

Determination of Contents of Psoralen and Isopsoralen in Yishenling Granules by RP-HPLC

目的:建立益肾灵颗粒中补骨脂素、异补骨脂素的含量测定方法。方法:采用反相高效液相色谱法,Diamonds色谱法,流动相为甲醇-水(48∶52),检测波长246 nm,流速为0.8 mL.min-1。结果:该方法的补骨脂素的回收率为98.94%(RSD=0.89%,n=5);异补骨脂素的回收率为100.27%(RSD=1.83%,n=5)。结论:本法简便、灵敏、准确,可用于本品补骨脂素、异补骨脂素的含量测定。

Objective： To establish a method for the determination of Psoralen and Isopsoralen in Yishenling Granules. Method： RPHPLC method was adopted with a Diamonds column. The mobile phase consisted of methanol and water（48： 52） with a flow rate of 0. 8 mL ·min^-1. A UV-detector at 246 nm was used. Results：The recoveries of Psoralen and Isopsoralen were 98.94% with RSD 0. 89% （n = 5） and 100. 27% with RSD 1. 83% （n = 5 ）, respectively. Conclusion： This method is simple, sensitive, accurate and can be used to control the quality of Yishenling Granules.