摘要
采用PCR-SSCP技术对蒙古羊BMP15基因外显子1位点的单核苷酸多态性(SNP)进行了检测,并对其与产羔性能关系进行了分析。结果表明,在所检测的116个蒙古羊个体中,BMP15基因在该位点均呈现多态性,具有++、A+和AA 3种基因型。测序分析表明,该段DNA序列中存在3个碱基的缺失,即编码区第28,29,30位碱基缺失,这个突变使野生型(++)氨基酸序列上相应的10号氨基酸残基亮氨酸(L)缺失,变成了突变基因型(AA),形成B1突变体;群体遗传学分析表明,B1突变在双羔系中的发生频率高于单羔系中发生的频率,χ2适合性检验结果表明,两品系在该位点具有中度多态性并处于Hardy-Weinberg平衡状态(p>0.05);与产羔性能关系分析表明B1突变对蒙古羊产羔数无显著影响(p>0.05)。
The single nucleotide polymorphism（SNP）at extronl site of BMP15 gene of Mongolian sheep was studied by PCR-SSCP and its association with prolificacy was analyzed. The results indicated that the BMP15 gene showed polymorphism in the 116 individuals and the genotypes ＋＋ , A ＋ and AA were found in Mongolian sheep. Sequencing analysis indicated that the 28th ,29th, 30th base in the encoding domain were lost in the DNA sequence, and the 10th amino acid residue of leucine（L）was lost by the mutation in the Wild Type（ ＋＋ ）amino acid sequence, forming the B1 mutant and the genotype was AA. Population genetic analysis indicated that the B1 mutation occurring in twins lamb strain was higher than in single lamb strain, 2 test indicated that the two strains were in moderate polymorphism and at Hardy-Weinberg equilibrium（p 〈 0.05）in this site. The BI mutation had no significant effect on the litter sixe by analyzed the association of the polymorphism with high prolificacy in Mongolian Sheep.
出处
《华北农学报》
CSCD
北大核心
2008年第5期30-34,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金(30560102)
