不同碳、氮营养对球孢白僵菌HFW-05的生长及胞外蛋白酶产量的影响

Effects of Different Carbon and Nitrogen Sources on the Growth and Extracellular Protease Production of Beauveria bassiana HFW-05

在基础培养基中添加14种碳源和11种氮源测定,球孢白僵菌HFW-05菌株的萌发率、生长速率、产孢量及胞外蛋白酶活性。综合各项指标得出,HFW-05菌株生长的最适碳源为蔗糖,产孢量与产酶量最大,分别为16.13×107孢子/cm2和117.38μg/(min.mL);最适氮源为豆粕粉,其产孢量和酶活远高于对照和无机盐类,分别为15.62×107孢子/cm2和74.01μg/(min.mL)。除硝酸钾外,无机盐类在供试浓度上均对孢子萌发和生长有抑制作用。在各种碳(氮)源培养基中添加1%蛋白胨(葡萄糖)后,菌株的产孢量和产酶量均有所提高。结果表明,HFW-05菌株的产孢量和胞外蛋白酶之间具有一定的相关性。

The spore germination rate, growth, sporulation and extracellular protease of Beauveria bassiana HFW-05 were investigated with 14 carbon sources and 11 nitrogen sources in media without any carbon and nitrogen nutrition. The results showed that the best carbon source was sucrose in which sporulation（16.13 ×10^7 conidia/cm^2） and extracellular protease （117.38 μg/（ min· mL））reached the highest. The bean cake was the best nitrogen nutrition in which the quantity of conidium（ 15.62 ×10^7 conidia/cm^2） and extracellular protease（74.01 μg/（min· mL） ） were much higher than that of the contrast and inorganic salts. All the inorganic salts except nitre restrained spore germination and growth in experiment conditions. The sporulation and extracellular protease were both enhanced by adding 1% peptone into carbon media and 1% glucose into nitrogen media. There were relationships among sporulation and extraeellular protease of B. bassiana.