胰岛素抵抗和肥胖不同易感性的关系研究

RESEARCH ON THE RELATIONSHIP BETWEEN INSULIN RESISTANCE AND SUSCEPTIBILITY OF OBESITY

目的研究高脂饲料饮食诱导肥胖不同易感性大鼠在形成胰岛素抵抗中的差异。方法健康雄性SD大鼠采用高脂饲料1(脂肪供能占45%)喂养3w后,按体重由高到低排序后分为三组:位于体重中间1/3的大鼠为对照组,改为喂饲基础饲料(脂肪供能占10%);位于体重上1/3的大鼠进入肥胖组,位于体重下1/3的大鼠进入肥胖抵抗组,此两组喂饲高脂饲料2(脂肪供能占60%)。分别于实验的3、6、8、10、11w末各组随机处死5只大鼠,动态监测各组大鼠体重、体脂含量、血脂、血糖、胰岛素和瘦素的变化趋势。结果肥胖组在能量摄入、体脂含量、血糖、血脂、血清胰岛素、胰岛素敏感指数和瘦素水平上都与肥胖抵抗组和对照组有显著性差异。结论在同样的高脂饲料诱导下,肥胖大鼠比肥胖抵抗大鼠更易形成胰岛素抵抗。

Objective To study the differences between diet induced obesity （DIO） and diet induced obesity resistance （DIO-R） during insulin resistance （IR） in rats. Method Male SD rats were fed by high-fat diet 1 （45 % fat kJ） after adaptation. At 3rd week, rats were divided into 3 groups according to their body weight： rats belonging to the middle 1/3 of their body weights were the control group, and was fed by control diet （10 % fat kJ）. The heaviest 1/3 and the lightest 1/3 belong to DIO and DIO-R group, respectively, and were fed by high-fat diet 2 （60 % fat k J）. Five rats were selected randomly from each group and sacarified at 3rd, 6th, 8th, 10th and 1 lth week. The energy intake, body fat contents, fasting blood glucose （FBG）, serum lipid, serum insulin, insulin sensitive index （ISI） and leptin of rats were monitored dynamically. Results DIO rats were significantly different from DIO-R and control rats in energy intake, body fat content, FBG, lipid, serum insulin and leptin. Conclusion Fed by the same high-fat diet, DIO rats I are more sensitive than DIO-R rats to become IR.