自体NK、T混合淋巴细胞扩增后回输对肿瘤免疫的影响

Effect of amplified autologous NK and T cells infusion from peripheral blood lymphocyte on tumor immunotherapy

目的:观察22例恶性肿瘤患者外周血淋巴细胞在体外经细胞因子扩增并回输前后肿瘤免疫状态的变化。方法:体外诱导扩增恶性肿瘤患者外周血淋巴细胞及单个核细胞,收获细胞后每2周回输1次,回输前用流式细胞术测定静脉血CD3+、CD3+CD4+、CD3+CD8+、CD3-CD16-CD56+、CD3-CD16+CD56-、CD3-CD16+CD56+、CD3+CD16-CD56+、CD3+CD16+CD56-、CD3+CD16+CD56+,观察回输前后上述指标的变化。结果:回输后2周外周血CD3+、CD3+CD8+、CD3+CD4+细胞与回输前相似;NK样标志细胞(CD16+/CD56+)比回输前明显增加(P<0.01);连续多次回输(2周间隔)后与上述结果相似;未见不良反应。结论:自体NK、T混合淋巴细胞(NK-Tm)扩增后回输可提高机体抗肿瘤细胞免疫功能,安全性良好。

Objective ： To study the change of blood lymphocyte phenotype after amplified autologous NK and T cells infusion from peripheral blood lymphocyte in 22 patients with cancer. Methods ： Mixture （named NKTm） of NK and T lymphocytes cells amplified from peripheral blood in malignant tumor patients was infused with 2 weeks interval. Before infusion and 2 weeks after infusion, the lymphocyte phenotype was detected by flow cytometry included CD3 ^＋ , CD3 ^＋ CD4 ^＋ , CD3 ^＋ CD8 ^＋ , CD3 CD16^-CD56 ^＋ , CD3CD16 ^＋ CD56^-, CD3CD16 ^＋ CD56 ^＋ , CD3 ^＋ CD16-CD56 ^＋ , CD3 ^＋ CD16 ^＋ CD56, CD3 ^＋ CD16 ^＋ CD56 ^＋. Results ： Two weeks after infusion, the phenotype of CD3 ^＋ , CD3 ^＋ CD8 ^＋ , CD3 ^＋ CD4 ^＋ cells did not significantly change, while the NK like cells （marked with CD16^＋/CD56^＋ ） statistically incresesd（P 〈0.01 ）. The results did not changed after multiple doses. No side-effects were found. Conclusion： Amplified autologous NK and T Cells infusion from peripheral blood lymphocyte may increase the antitumor immune without any side-effects.