流感嗜血杆菌体外生物被膜的形成

Bacterial biofilm formed by haemophilus influenzae in vitro

目的:观察流感嗜血杆菌体外生物被膜(bacterial biofilm,BBF)的形成。方法:分离慢性阻塞性肺病急性加重(acute exacerbation of chronic obstructive pulmonary disease,AECOPD)患者痰液中的流感嗜血杆菌11株,采用结晶紫法和扫描电镜方法研究BBF的形成和结构。结果:不同菌株流感嗜血杆菌体外形成BBF的能力有所不同,超微结构显示由细菌菌体和胞外基质形成典型的膜状结构。结论:流感嗜血杆菌可在体外形成稳定的BBF,提示与AECOPD的发病有关。

Objective： To observe bacterial biofilm formed by Haemophilus Influenzae in vitro. Methods： 11 strains of Haemophilus Influenzae were isolated from sputum collected from patients with AECOPD. Formation of bacterial biofilm was examined by crystal violet assay and scanning electron microscope. Results： Striking differences were observed among strains with regard to the ability to form biofilm. Typical membrane-like structure formed by bacterial cells and extracellular matrix was detected. Condusion： Haemephilus Influenzae is capable to form bacterial biofilm in vitro, which related with pathogenesis of AECOPD.