尿脱落细胞hTERT mRNA表达在早期膀胱癌无创诊断上的探索

Investigation on early and non-hurt diagnosing bladder cancer by expressing of hTERT mRNA of exfoliated urorhelial cells

目的:通过检测尿脱落细胞的hTERT mRNA和端粒酶的活性,探讨建立膀胱癌早期诊断和术后检查的无创方法。方法:利用可提高灵敏度的巢式PCR技术,对尿脱落细胞中hTERT的mRNA进行RT-PCR检测,以TRAP方法加以验证。结果:28例膀胱癌患者尿脱落细胞hTERT的mRNA表达阳性率为93%(26/28)。20例泌尿系良性疾病组患者则为10%(2/20),TRAP方法有很好的一致性。结论:尿脱落细胞hTERT mRNA的检测敏感性高,特异性好,有望应用于膀胱癌的早期诊断和术后复发的无创检查。

Objective： To study the mRNA of human telomerase reverse transcriptase （hTERT） in exfoliated urinary cells of bladder cancer and to evaluate its clinical significance. Methods： We examined the expression of mRNA of hTERT in exfoliated urorhelial cells of bladder and control by RT-PCR. Meantime, the telomere reoeat amplification protocol （TRAP） was performed for indentify the result of mRNA of hTERT. Results： The positive rate of hTERT mRNA of bladder cancer group is 93% （26/28） and control group is 10% （2/20）. The result was consistent with the TRAP. Conclusion： The result suggest the expression of hTERT mRNA can be useful in screeing for bladder cancer and diagnosing bladder cancer recrudesce after operation.