芳香化酶抑制剂Formestane和Letrozole对番鸭性腺分化的研究

Aromatase inhibitor of formestane and letrozole on sexual differentiation of muscovy

在番鸭胚性腺未分化前注入不同类型的芳香酶抑制剂Formestane(类固醇型)和Letrozole(非类固醇型),研究对其性腺分化的影响。结果表明,在Formestane 3个处理组中,50μg.枚-1处理组公母雏鸭性别比差异不显著;100和150μg.枚-1处理组对番鸭雄性化作用较强,雄性比差异极显著(P<0.01),经遗传性别鉴定,性反转率分别为52.94%和55.56%,但是随着浓度的加大番鸭胚的孵化率逐渐降低。在Letrozole的3个剂量组中,100μg.枚-1处理组雄性比差异显著(P<0.05),性反转率为36.36%,而50和150μg.枚-1处理组公母雏鸭性别比差异均不显著(P>0.05),此外,3个处理组均显著降低了番鸭胚的孵化率。对性反转番鸭性腺进行解剖组织学观察,性反转完全番鸭的两侧性腺似睾丸组织,而性反转不完全番鸭的两侧性腺存在不同程度的反转,一般左侧性腺反转程度低于右侧,组织结构介于睾丸与卵巢之间。

The differentiation of poultry′s gonads is related to the regulation of estrogen,and the synthesis of estrogen is catalyzed by aromatase.The female muscovy gonads can develop into male when the activity of aromatase is blocked by aromatase inhibitor before sexual differentiation.In order to learn the effection of aromatase inhibitor to the hatchability and the growth of sexual gonade on muscovy,in this experiment,the fertilized eggs were injected separately with two kinds of aromatase inhibitors,formestane（steroid） and letrozole（nonseroid）.The results showed that 100 and 150 μg·egg-1 formestane（steroid） can effectively reverse female muscovy to male（P〈0.01）,the rate of masculinization was 52.94% and 55.56% which was identified by genetic sex,but the hatchability of muscovy decreased along with the concentration of formestane,100 μg·egg-1 letrozole（nonseroid） can effectively reverse female muscovy to male（P〈0.05） and the rate of masculinization was 36.36%,but 50 and 150 μg·egg-1 letrozole can′t reverse sexual efficiency.The hatchability of letrozole groups were lower than that of the normal group.The two sides of sexual gonads on fully masculinized muscovys were like normal testis,but there were some morphologic differentiations among the two sides of sexual gonads on partial masculinized muscovys.The left gonad was reversed less than the right′s and the structure was between testis and ovary.