沼液样品静置富集培养及好氧纤维素降解菌的分离

Isolation of aerobic cellulolytic strain from enrichment of biogas slurry

好氧纤维素降解菌,能够促进沼气系统启动阶段厌氧条件的建立以及木质纤维素的降解,因此对于加速沼气系统产气具有重要的作用。通过沼液的静置富集培养,使用选择性培养基进行分离,分离到了1株适合于沼液环境的好氧纤维素降解菌。对其16S rRNA基因进行测序,结果表明这株菌属于放线菌中的链霉菌属。最后通过克隆该株菌的ER IC-PCR指纹图谱条带,找到了1段该菌株特异性序列,能够为监测该菌在沼气强化过程中的应用提供适合的分子标记。

Aerobic cellulolytic bacteria might facilitate formation of anaerobic condition for methanogenesis and degradation of lignocellulosic material at start-up stages of biogas fermentation, so they are important for bioaugmen- tation of biogas production. In this study, aerobic cellulolytic bacteria were enriched by static culture of biogas slur- ry and subsequently, one aerobic cellulolytic strain was isolated. The strain belongs to genus of Streptomyces according to 16S rRNA gene sequence. At last, a molecular,marker of the strain was obtained by cloning one band of ERIC-PCR fingerprinting, which could be used for monitoring the strain in biogas fermentation.