HPLC法测定芎菊上清丸中黄芩苷的含量

Determination of baicalin in Xiongju Shangqing Pills by HPLC

目的：建立高效液相色谱法测定芎菊上清丸（大蜜丸）中黄芩苷含量的方法。方法：色谱柱为Waters Spheri-sorb ODS2柱（4．6mm×250mm，5μm），流动相为甲醇-水-磷酸（46：54：0．2），流速0．8ml／min，检测波长277nm。结果：黄芩苷在4．960×10^-3～0．248mg／ml浓度范围内呈良好的线性关系；平均回收率为96．6％，RSD为1．1％。结论：本方法简便、准确，可用于芎菊上清丸的质量控制。

OBJECTIVE To develop an HPLC method for the determination of baicalin in Xiongju Shangqing Pills. METHODS The separation was performed on a ODS column（4.6 mm×250 mm,5μm） with methanol - water - phosphoric acid（46： 54： 0.2） as the mobile phase. The wavelength of detection was 277 nm and the flow rate was 0.8 ml/min. RESULTS There was a good linearity over the concentration range of 4. 960×10^-3 -0.248 mg/ml, the average recoveries was 96.6% with relatively standard deviations of 1.1%. CONCLUSION This method is simple accurate and stable, it can be used for the quality control of Xiongju Shangqing Pills.