基质金属蛋白酶-9及其抑制剂与肝癌侵袭转移的关系

Expression of matrix metalloproteinase-9 and tissue inhibitor in hepatocellular carcinoma and its relationship with invasion and metastasis.

目的研究基质金属蛋白酶-9(MMP-9)及其组织抑制剂-2(TIMP-2)在肝细胞肝癌(HCC)组织中的表达及其与HCC侵袭转移的关系。方法应用组织芯片技术和免疫组化方法(SP法)检测93例HCC组织、66例癌旁组织和36例正常肝组织中MMP-9和TIMP-2的表达情况。结果MMP-9在HCC癌组织中的表达阳性率(62.4%)和癌旁组织(57.6%)高于正常肝组织(11.1%,P<0.001);MMP-9的表达随癌的转移而升高(P<0.01)。TIMP-2在HCC中表达阳性率(51.6%)低于癌旁组织(83.3%,P<0.001)和正常肝组织(86.1%,P<0.001);TIMP-2表达随癌的转移而降低(P<0.05)。MMP-9和TIMP-2与患者合并硬化、组织分化、AFP水平和HBsAg状态无关(P>0.05)。结论MMP-9和TIMP-2表达与HCC的侵袭转移密切相关,MMP-9和TIMP-2可以作为临床判断HCC侵袭转移有价值的参考指标。

Objective To study the expression of matrix metalloproteinase - 9 （ MMP - 9） and tissue inhibitor of matrix metalloproteinase- 2 （TIMP- 2） in hepatocellular carcinoma（ HCC ）and their relationship with invasion and metastasis. Methods The expression of MMP - 9 and TIMP - 2 was detected with tissue microarray by immunohistochemieal method in 93 HCC tissues,66 adjacent nontumor tissues and 36 normal liver tissues. Results The expressions rate of MMP -9 in HCC tissues （62.4%） and that adjacent nontumor tissues （57.6%） was significantly higher than normal liver tissues （11. 1%, P 〈 0.001 ） ;it become higher associated with the increasing of invasion and metastasis of HCC （P 〈 0.01 ）. The expressions rate of TIMP-2 in HCC tissues （51.6%） was significantly lower than that adjacent nontumor tissues （83.3%, P 〈 0. 001 ）and normal liver tissues （86.1% , P 〈 0. 001 ） ; it become lower associated with the increasing of invasion and metastasis of HCC （P 〈 0.05 ）. The expression of TIMP -9 and TIMP -2 was no correlation with cirrhosis, Edmondson, s histological grade of carcinoma , AFP levels and HBsAg situation （ P 〉 0.05 ）. Conclusion The expressions of MMP - 9 and TIMP - 2 are significantly correlated with the invasion and metastasis of HCC. They may serve as indicators for determining the metastasis in HCC.