草灵芝对乙醇性肝损伤的保护作用

Effect of Cao Lingzhi on protecting against alcoholic liver injury

目的探讨草灵芝对酒精性肝损伤的保护作用。方法将受试大鼠70只随机分成5个组,每组14只。应用乙醇建立大鼠肝损伤模型,测定肝组织的丙二醛(MDA)、甘油三酯(TG)及还原型谷胱甘肽(GSH)含量;观察肝脏的病理组织学改变。结果受试物高、中、低各组的MDA均值分别为0.56,0.58,0.53nmol/g(肝组织),均低于肝损模型组的0.63nmol/g;血清甘油三酯含量均值分别为2.27,3.42,3.15mmol/L(血清),均显著低于肝损模型组的4.40mmol/L;肝组织甘油三酯含量均值分别为3.04,3.59,3.83mmol/100g(肝组织),均低于肝损模型组的3.86mmol/100g;而还原型谷胱甘肽含量均值分别为26.5,18.6,23.8μmol/g(肝组织),均显著高于肝损模型组的13.8μmol/g;各剂量组大鼠肝脏病理改变评分均值分别为155.4,192.3,194.6分,均显著低于肝损模型组的260.8分。结论草灵芝对乙醇性大鼠肝损伤具有保护作用。

Ojective To explore the protective effect of Cao Lingzhi on rat model of liver injury caused by alcohol. Methods Seventy rats were divided into 5 groups randomly, 15 rats per group. Rat models of alcoholic liver injury were established. The contents of malondialdehyde （MDA）, triacylglycerol （TG） and reduced glutathione （GSH） in liver tissue were measured, and the pathological and histological changes were observed. Results The MDA contents in liver tissue in the high, middle, and low dosage groups were 0.56, 0.58, and 0. 53nmol/ g, respectively, lower than that in the model control group（0.63nmol/L） .The TG contents in serum in the high, mid- dle, and low dosage group were 2.27, 3.42, and 3.15mmol/L respectively, significantly lower than that in the model control group（4.40mmol/L） .The TG contents in liver tissue in the high, middle, and low dosage groups were 3.04, 3.59, and 3.83mmol/100g, lower than that in the model control group （3.86mmol/100g）, while the GSH contents in liver tissue in the high, middle, and lower dosage groups （26.5, 18.6, 23.8μmol/g）were significantly higher than that in the model group（ 13.8μmol/g）. The average value of pathological changes in rat liver in the high, middle, and low dosage group were 155.4, 192.3, and 194.6 respectively, significantly lower than that in the model control group （260.8）. Conclusion Cao Lingzhi has a significant protective effect on liver injury caused by alcohol.