从催吐萝芙木中提取利血平和育亨宾的工艺研究

Purification Process of Reserpine and Yohimbine from Rauvolfia vomitoria

研究从催吐萝芙木中提纯利血平和育亨宾的工艺。催吐萝芙木根粉用酸性乙醇水溶液浸提,正交实验法确定浸提条件为:浸提2次、每次3 h、浸提剂含50%乙醇0、.05 mol/L硫酸。浸提液经HZ-818大孔吸附树脂吸附,用40%乙醇溶液(含1%HCl)解吸育亨宾,用80%乙醇溶液(含1%HCl)解吸利血平,育亨宾和利血平得到完全分离。两种解吸液分别经过沉淀、结晶,得到利血平和育亨宾。所得利血平纯度为99.8%,总收率为27.1%;育亨宾纯度为4.9%,总收率为9.8%。本工艺能从催吐萝芙木中提取利血平和育亨宾,有利于充分利用中药材,降低成本。

A purification process of reserpine and yohimbine from Rauvolfia vomitoria was investigated. The root powder of Rauw）lfia vomitoria was extracted with acidic alcohol solution. The optimum conditions from orthogonal experiments were： extracting two times, each time for 3 h, and extracting liquid containing 50 % alcohol and 0.05 mol/ml H2SO4. Extracting liquid was adsorbed by HZ-818 macroporous resin. Yohimbine was eluted with 40% alcohol solution and reserpine was eluted with 80% alcohol solution. Reserpine and yohimbine were separated thoroughly. These two kinds of elutes were purified respectively through a series of processes in cluding precipitation and crystallization, reserpine and yohimbine were obtained. The purity of reserpine was 99. 8% and the recovery rate was 27.1 %. The purity of yohimbine was 4. 9% and the recovery rate was 9.8%. The process could purify reserpine and yohimbine from Rauvolfia vomitoria. It is beneficial to utilize the medicinal materials fully and lower the production cost.