内部沸腾法提取分离葛根中的葛根异黄酮和大豆异黄酮

Extraction-purification of pueraia isoflavone and soyisoflavones from Radix Puerariae lobatae by inner ebullition

目的:采用正丁醇为提取溶剂的内部沸腾法提取葛根异黄酮和大豆异黄酮。方法:用少量乙醇溶液润湿葛根粉末使其中的葛根异黄酮充分解吸,然后加入一定温度的含水正丁醇溶液,使渗透到葛根组织内部的乙醇沸腾,强化提取过程。由于大豆异黄酮与葛根异黄酮在丁醇中的溶解度有较大差异,因此浓缩过程中可采用分步蒸发浓缩结晶,浓缩除去部分溶剂后,结晶分离出大豆异黄酮,最后蒸干结晶母液,得到葛根异黄酮。结果:在80mL60%乙醇、解吸时间20min、含水量20%的600mL正丁醇温度90℃时,总黄酮得率可达到11.3%。当浓缩除去90%的溶剂时,大豆异黄酮与葛根异黄酮的质量比为1∶3。经液相检测,葛根异黄酮中葛根素含量可达46.0%;大豆异黄酮中大豆苷的含量为6.12%。结论:内部沸腾提取及溶剂蒸发结晶分离的工艺简单,为从葛根中同时提取大豆异黄酮与葛根异黄酮的工业化生产提供了依据。

AIM： To extract pueraia isoflavone and soyisoflavones with n-butanol by means of inner ebullition from Radix puerariae lobatae. METHODS ： A little ethanol was first used to saturate the powder of Radix puerariae lobatae to make the pueraia isoflavone desorbed adequately, and then hot n-Butanol solution was added to make the ethanol inside Radix puerariae lobatae begin to boil and the extraction was intensified. Based on different solubility of pueraia isoflavone and soyisoflavones in n-Butanol, when solvent vaporized, the soyisoflavones were separated and then the pueraia isoflavone by step vaporizeing on centrating crystal. RESULTS： 80 mL of 60% ethanol, saturating time 20 min, 600 mL n-Butanol including 20% water, at the temperature of 90℃, the yield of total isoflavone was 11.3 %. When 90% solvent vaporized, the ratio of quality of the soyisoflavones and pueraia isoflavone was 1： 3. The puerarin and daidzin＇s purity reached 46. 0% and 6. 12% by the HPLC respectively. CONCLUSION ： The technology of inner ebullition extraction and solvent vaporizing crystal separation are simple,which provide industry production basis for pueraia isoflavone and soyisoflavones.