瓜馥木化学成分的研究(Ⅲ)

The chemical constituents of Fissistigma oldhamii(Ⅲ)

目的:研究瓜馥木的化学成分。方法:采用硅胶柱色谱技术进行分离纯化,运用波谱法进行了结构解析,并进行了体外细胞毒活性评价。结果:从瓜馥木根的乙醇提取物中分离得到5个化合物,经理化常数和波谱(EI-MS、1H-NMR、13C-NMR)分析,鉴定为马兜铃内酰胺AⅡ(Ⅰ)、马兜铃内酰胺BⅡ(Ⅱ)、大黄素甲醚(Ⅲ)、β-谷甾醇(Ⅳ)及豆甾烷-7-酮(Ⅴ);化合物Ⅰ对肺腺癌GLC-82及HL60白血病细胞株均具有细胞毒作用。结论:化合物Ⅲ～Ⅴ为首次从瓜馥木植物中分离得到,化合物Ⅰ对肺腺癌GLC-82、白血病细胞株HL60的细胞株的IC50分别为:234.45、101.17μM。

AIM： To study the chemical constituents of Fissistigma oldhamii （Hemsl.） Merr. METHODS： Silica gel column chromatography was used in the isolation procedure, the structures of the isolated compounds were elucidated by spectral data. Meanwhile cytotoxic activity of compound Ⅰ was made according to the cell experiment in vitro. RESULTS ： Five compounds were isolated from Radix of Fissistigma oldhamii （ Hemsl. ） Merr.. On the basis of physical-chemical constants and spectral data （EI-MS, ^1H-NMR, ^13C-NMR）, these compounds were identi- fied as： Aristolactam A Ⅱ （Ⅰ ）, Aristolactam B（ Ⅱ ）, Physcion（ Ⅲ ）, β-sitosterol（ Ⅳ）, Stigmastan-7-one（ Ⅴ ）.Compound Ⅰ showed cytotoxic activities on GLC-82 and HL60 cell strains. CONCLUSION： Compounds Ⅲ, Ⅳ and Ⅴ are firstly isolated from Fissistigma oldhamii （Hemsl.） Merr.. The IC50 of compound Ⅰ on GLC-82 and HL60 cell strain are： 234.45, 101.17 μM.