摘要
目的通过研究CLC-3在人右心房的表达特点及影响表达的相关因素,探讨CLC-3蛋白与容量敏感的氯离子电流(ICl.vol)的关系。方法取33例心脏手术患者右心房组织,半定量RT-PCR检测CLC-3mRNA;选取可能影响CLC-3mRNA表达的相关因素如性别、年龄、是否房颤、右房横径进行多元线性回归分析;免疫组化检测CLC-3蛋白的表达,并观察CLC-3蛋白在细胞中的分布。结果RT-PCR和免疫组化均可检测到CLC-3的表达,且CLC-3蛋白分布在细胞内;多元线性回归分析提示CLC-3mRNA的表达与性别、年龄、是否房颤、右房横径没有相关性。结论CLC-3可能不是介导ICl.vol的通道蛋白。
Objective To explore the relationship between CLC-3 and ICl. vol by investigating the characteristic and correlativity of the expression of CLC-3 in human right atrium. Methods The right atrium was obtained from 33 patients undergoing cardiac surgery. The expression of CLC-3 mRNA was semi-quantitatively detected by using RT-PCR. The factors （gender, age, atrial fibrillation and transverse diameter of right atrium） possibly influencing the expression of CLC-3 mRNA were analyzed by the multiple linear regression method. The expression and the location of CLC-3 protein were assayed by using immunohistochemistry. Results The expression of CLC-3 was detectable, and CLC-3 distributed in the cytoplasm. There was no correlation between the expression of CLC-3 mRNA and gender, age, atrial fibrillation or transverse diameter of right atrium. Conclusion CLC-3 may not be a channel protein mediating ICl. vol.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2008年第5期633-636,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
教育部留学回国人员科研启动基金资助项目(No.教外司留[2004]527号)
