摘要
目的建立空心莲子草正丁醇部位的高效液相色谱(HPLC-ELSD)指纹图谱。方法HPLC-ELSD法测定,流动相为乙腈-水梯度洗脱,流速为1.0 ml.min-1,蒸发光散射(ELSD)检测器,柱温35℃。结果运用梯度洗脱能较好的分离空心莲子草正丁醇部位化合物;经方法学考察,HPLC-ELSD指纹图谱具有良好的重复性,初步建立了以12个共有峰为特征指纹信息的HPLC-ELSD指纹图谱。结论建立的空心莲子草正丁醇部位的HPLC-ELSD指纹图谱分析法可为空心莲子草的质量评价提供参考。
Objective To establish the HPLC - ELSD of n - butylol froution of Altemanthera philoxeroide. Methods The gradient elution was adopted with a mixture consisting of acetonitrile and water, the flow rate was 1.0 ml · min^ -1, and the detector was ELSD. Results The constituents of Altemanthera philoxeroide were well separated on HPLC and the HPLC -FPC of 10 batches of samples was established. The results showed that this method had a good repeatability and the 12 common peaks had an exclusive properties. Conclusion The method developed can supply the reference for the quality evaluation of Altemanthera philoxeroide.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第10期2330-2332,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30470194)
