高效液相色谱法测定五虎散中羟基红花黄色素A的含量

Determination of Hydroxysafflor Yellow A in the Wuhu Powder by HPLC

目的建立复方制剂五虎散中红花有效成分的高效液相色谱测定方法。方法采用Agilent C18（5μm，250mm×4．60mm）色谱柱；以甲醇-0．4％磷酸水（33：67）为流动相，流速为1ml·min^-1，检测波长为402nm。结果羟基红花黄色素的线性范围分别为0．0565—0．452μg（r=0．9994），平均加样回收率（n=6）为99．15％，RSD为4．51％。结论该测定方法简便可行、重复性好，可用于该制剂中红花有效成分的含量测定。

Objective To establish the method for determination of effective components in Wuhu powder by RP - HPLC. Methods The contents of hydroxysafflor yellow A was determined by an HPLC system using following conditions ： the column was Agilent C18（5 μm, 250 mm ×4.60 mm） , with CH3OH -0.4% H3PO4water solution（33：67） as mokile phase, the flow rate was 1.0 ml · min ^-1 , the detection wavelength was at 402 nm. Results The linear range of hydroxysafflor yellow A was 0. 056 5 -0. 452 μg（ r = 0. 999 4） ; its average recovery were 99.15% , corresponding RSD were 4.51%. Conclusion The HPLC method is simple, accurate, highly selective and reproducible, thus it can be used as quality control of Compound Wuhu powder.