PCR-DGGE技术评价抗生素诱导的肠道菌群失调

Assessment of antibiotic-induced intestinal flora imbalance by PCR-DGGE

目的利用PCR-DGGE技术结合活菌计数评价抗生素引起的肠道菌群失调。方法SPF级BALB/c雌性小鼠连续4d灌胃头孢曲松溶液125mg/ml,0.4ml/d,运用基于细菌16S DNA的PCR-DGGE技术结合活菌计数分析小鼠肠道菌群变化。结果活菌计数结果与PCR-DGGE图谱显示,头孢曲松处理3d后小鼠肠道菌群出现严重失调。结论PCR-DGGE技术可直观而灵敏地显示抗生素处理过程中肠道菌群的动态变化,适用于评价抗生素引起的肠道菌群失调。

Objective To assess the ceftriaxone-induced intestinal flora imbalance by PCR-DGGE method and plate count. Methods Female SPF BALB/c mice were treated with ceftriaxone in a dose of 125 mg/ml,0.4 m//d by oral gavage for four days. The amount of fecal dominant bacterium were quantified by viable count, and diversity of microflora was detected by 16S DNA-based PCR-DGGE. Results Both plate count and PCR-DGGE profile indicated that dysbacteria could be induced by administering cefiriaxone continually for 3 days. Conclusion PCR-DGGE can directly and sensitively display the dynamic succession of mice intestinal flora during the antibiotic treated process, which makes it an effcient method to assess the antibiotic-induced intestinal flora imbalance.