鹅副粘病毒WF_(00)G分离株HN蛋白基因的测序与序列分析

Sequencing and sequencing analysis of HN gene of goose paramyxovirus WF_(00)G strain

用鹅副粘病毒凤阳分离株WF00G做9～10日龄SPF鸡胚的尿囊腔接种，成功增殖了该病毒，采用一步法RT-PCR技术扩增WF00G病毒的HN基因，获得了1条约1．8kb的特异性条带。PCR产物回收纯化后测序。测序结果表明，扩增片段大小为1844bp，含有1个1716bp的开放性阅渎框，编码571个氨基酸。核苷酸同源性分析表明：WF00G株与其他12株鹅副牯病毒的同源性为89．9％-99．2％，与国内外其他NDVHN基因的同源性为81．7％～95．7％，其中与国内标准强毒株F48E9的同源性为84．7％，说明WF00G与国内外的传统毒株有较大变异。与Taiwan95株和NL／96株的同源性为94．3％和95．7％，说明WF00G与Taiwan95株和NL／96株亲缘关系较近，具有较高的相似性。

The goose paramyxovirus of WF00G strain was grown in 10-day-old SPF embrocated eggs. The HN gene of WF00G was amplified by one step RT-PCR. The sequencing analysis showed that the sequence of the HN gene was 1844 bp and encoding a protein of 571 amino acids. The homology between WFooG and the other goose paramyxovirus strains was 89.9% to 99.2%. The homology between WF00G and the NDV strains isolated inside and outside China was from 81.7% to 95.2%. The WF00G HN gene＇s nueleotide sequence share 84.7% homology with that of the NDV strain F48E9. The ORF of WF00G HN gene shared 94.3% and 95.7% nucleotide homology with that of the NDV strain Taiwan95 and NL/96 respectively. The results indicated the mutation on the HN gene had varied largely, compared with classic NDV.