鸦胆子油乳对人肺癌A549细胞血管内皮生长因子表达的影响

Effects of brucea javanica oil on expression of vascular endothelial growth factor in A549 cell line

目的:研究鸦胆子油乳对人肺癌A549细胞血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法:以健康人外周血单个核细胞(peripheral polymorphonuclear neutrophil,PMN)为正常对照,分别用不同剂量的鸦胆子油乳(0.5,1.25,2.5,5 g.L-1)与人肺癌A549细胞共育48 h,采用定量sandwich酶免疫技术和RT-PCR法分别测定PMN细胞和A549细胞培养上清液VEGF分泌和细胞内VEGF mRNA的表达,未加药组采用等量RPMI 1640培养液。结果:A549细胞上清液VEGF分泌量较外周血单个核细胞显著上升(120.73 vs21.21,P<0.05),2.5 g.L-1鸦胆子油乳作用48 h能使A549细胞VEGF分泌显著减少(20.30 vs 120.73,P<0.05)。A549细胞VEGF mRNA表达较骨髓单个核细胞也显著上升(0.957 3 vs 0.464 9,P<0.05),鸦胆子油乳(0.5,1.25,2.5 g.L-1)作用48 h对A549细胞VEGF mRNA表达无显著影响,5 g.L-1鸦胆子油乳可显著降低A549细胞VEGF mRNA表达(0.468 2 vs 0.957 3,P<0.05)。结论:鸦胆子油乳能降低A549细胞VEGF的分泌和表达,该作用可能是其抗肿瘤的作用机制之一。

Objective： To study the effects of brucea javanica oil on the expression of vascular endothelial growth factor （YEGF） in A549 cell line. Method： A549 cells were incubated with different concentrations of brucea javanica oil （0. 5, 1.25, 2. 5, 5 g · L^-1） for 48 h respectively. VEGF level in supernatant was determined by VEGF ELISA kits and mRNA expression of VEGF was evaluated by RT-PCR. PMN in health volunteers was treated as control groups. Result： Supernatant VEGF protein and mRNA expres- sion were significantly elevated in A549 cells compared with the mononuclear cells （ 120.73 vs 21.21, P 〈0. 05 ）. Brucea javanica oil （2.5 g · L^-1 ） could reduced supernatant VEGF protein in A549 cells （20. 30 vs 120. 73, P 〈0. 05）, but had no effect on the expres- sion of VEGF mRNA （ 1. 023 0 vs 0. 957 3 ）. It was found that brucea javanica oil （5 g · L^-1 ） significently reduced VEGF mRNA expression （0. 468 2 vs 0. 957 3, P 〈 0.05 ）. Conclusion： Brucea javanica oil can depress the VEGF mRNA expression and secretion in A549 cells, which may be one of the mechanisms of its antitumor effect.